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在孟加拉国Trichoderma asperellum的分离,形态生理和分子表征,植物遗传学分析

S A Uddin¹, I Hossain², H Mahmud³

¹Shyamnagar Govt. Mohsin Degree College, Shyamnagar, Satkhira, Bangladesh.

Brazilian journal of biology = Revista brasleira de biologia

|December 4, 2024

概括

这项研究确定了孟加拉国土壤中的Trichoderma asperellum,并证实了其对像Fusarium oxysporum这样的植物病原体的有效性. 分子分析验证了分离物,突出了它们在农业中的生物控制潜力.

科学领域:

植物病理学植物病理学
农业微生物学农业微生物学
分子生物学分子生物学

背景情况:

土壤传播的植物疾病对全球的作物生产构成重大威胁.
生物防治剂为化学农药提供了一个可持续的替代品.
特里科德玛阿斯佩雷勒 (Trichoderma asperellum) 是一种已知的有益真菌,具有治疗疾病的潜力.

研究的目的:

从孟加拉国的各种农业土壤中选和识别Trichoderma asperellum分离物.
评估已识别的T. asperellum分离物对关键的土壤传播植物病原体的体外对抗潜力.
用分子技术确认选择的T. asperellum分离物的身份.

主要方法:

从孟加拉国107个地点的49种作物中采集了球土壤样本.
使用形态和生理学表征来选择15个T. asperellum分离物.
对抗性活性被测试在Fusarium oxysporum,Sclerotium rolfsii和Pythium aphanidermatum之间.
分子鉴定涉及ITS和TEF区域的测序,以进行遗传学分析.

主要成果:

十五个T. asperellum分离物被净化和特征化;两个 (TR27,TR45) 在40°C时分泌.
T. asperellum分离物表现出显著的对抗作用,减少了脚/根腐烂,领腐烂和缓解疾病.
包括ITS和TEF测序在内的分子分析证实了四个关键分离物的身份 (TR27,TR45,TR70,TR85) 与T. asperellum具有很高的同质性.
使用Tef1区域的家族遗传学分析提供了与ITS区域相比,Trichoderma分离物之间更好的分化.

结论:

来自孟加拉国的Trichoderma asperellum分离物具有控制主要土壤传播植物疾病的巨大潜力.
分子表征,特别是使用Tef1区域,对于准确识别和区分Trichoderma物种是有效的.
这些发现支持开发基于T. asperellum的生物控制策略,用于孟加拉国的可持续农业.

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