时钟转录的替代拼接介绍了昼夜时钟对温度变化的反应
Yao D Cai1, Xianhui Liu2, Gary K Chow1
1Department of Entomology and Nematology, College of Agricultural and Environmental Sciences, University of California Davis, Davis, CA 95616.
概括
温度变化会影响昼夜时钟. 在Drosophila中,低温会产生一个CLK变体 (Clk-cold),缺少一个关键的酸化部位,增加了时钟基因活性和季节性适应.
科学领域:
- 时间生物学 时间生物学
- 分子生物学分子生物学
- 遗传学 遗传学 是一个
背景情况:
- 循环时钟调节每天的生物节奏,适应季节性温度变化,以获得最佳的身体健康.
- 在Drosophila中,周期 (per) 和永恒 (tim) 基因的温度敏感替代拼接 (AS) 对于季节性适应至关重要.
- 钟 (Clk) 基因是昼夜基因表达的关键激活剂,此前尚未对温度敏感的AS.研究过.
研究的目的:
- 为了研究Drosophila.的时钟 (Clk) 基因对温度敏感的替代拼接 (AS).
- 确定一种新的Clk转录变体 (Clk-cold) 对生理时钟调节的功能后果.
- 阐明AS,酸化和温度反应在昼夜时钟中的相互作用.
主要方法:
- 在不同的温度条件下分析Clk转录变异.
- 描述CLK冷蛋白异型及其特性.
- 调查CLK酸化在血清13 (S13) 和其对DNA结合和转录活性的影响.
- 测试以确定时钟基因和转录输出中的CLK占用率.
- 研究PER在CLK酸化中的作用的实验.
主要成果:
- 德洛索菲拉Clk转录表现出对温度敏感的AS,在低温下产生一种Clk-cold变体.
- 该CLK冷蛋白缺乏四种氨基酸,包括CK1α依赖酸化位点S13.
- 在CLK ((S13) 的酸化降低了CLK的DNA结合和转录活性;冷CLK缺乏这种调节部位.
- 低温导致时钟基因的CLK占用率增加和转录输出量增加,这归因于较高的Clk-cold水平.
- PER促进了CLK的CK1α-依赖的酸化,这表明抑制剂的酶-支架作用是保留的.
结论:
- 产生CLK冷异形的Clk的温度敏感AS是Drosophila季节性适应的关键机制.
- AS和调节之间的相互作用根据环境温度对CLK活动进行微调.
- 这项研究揭示了一个保留的调节模块,涉及AS和酸化在真核生物昼夜钟.
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