相关实验视频

Updated: Jun 5, 2025

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Chromatin Immunoprecipitation Assay for the Identification of Arabidopsis Protein-DNA Interactions In Vivo

Published on: January 14, 2016

B盒蛋白CmBBX8招募染色体修饰剂CmFDM2/CmSWI3B,以诱导夏季花的开花

Qi Wang¹, Chaona Si¹, Qingling Tang¹

¹State Key Laboratory of Crop Genetics and Germplasm Enhancement and Utilisation, Key Laboratory of Landscaping, Ministry of Agriculture and Rural Affairs, Key Laboratory of Biology of Ornamental Plants in East China, National Forestry and Grassland Administration, Zhongshan Biological Breeding Laboratory, College of Horticulture, Nanjing Agricultural University, Nanjing, China.

The Plant journal : for cell and molecular biology

|December 4, 2024

概括

夏季花的开花包括CmBBX8激活CmFTL1. 这项研究显示,CmBBX8招募了CmFDM2和CmSWI3B染色体修饰剂,在CmFTL1位点减少H3K27me3,以促进花过渡.

科学领域:

植物生物学植物生物学
分子遗传学分子遗传学
表观遗传学在表观遗传学中,表观遗传学是指表观遗传学.

背景情况:

植物的开花是由从植物生长过渡到生殖生长的过程调节的.
在夏季花中,蛋白质CmBBX8激活了CmFTL1的表达,这是花过渡中的关键基因.

研究的目的:

阐明CmBBX8在香花的光周期性花诱导过程中诱导CmFTL1表达的机制.
研究CmFDM2和CmSWI3B在CmBBX8介导的开花途径中的作用.

主要方法:

酵母两杂交测试以确定蛋白质相互作用.
在花中,CmFDM2和CmSWI3B的过度表达和降低.
染色体免疫沉 (ChIP) 试验分析基因促进体结合和基因素修饰.
定量实时PCR (qRT-PCR) 用于测量基因表达水平.

主要成果:

CmBBX8与类似SGS3的蛋白质CmFDM2以及染色体重塑因子CmSWI3B相互作用.
过度表达CmFDM2导致提前开花,而其下降导致延迟开花.
CmFDM2与CmFTL1促进体结合,激活其表达并部分依赖CmSWI3B.
通过CmBBX8介导的开花诱导依赖于CmFDM2和CmSWI3B.
在CmBBX8,CmFDM2和CmSWI3B招募时,在CmFTL1位点观察到降低的H3K27me3水平,与增加的CmFTL1转录水平相关.

结论:

CmBBX8通过招募染色质修饰剂CmFDM2和CmSWI3B来促进花的开花.
这种招募导致CmFTL1位点的H3K27me3减少,促进其表达和花过渡.
这些发现为对光周期的反应中花诱导的表观遗传调节提供了新的见解.

关键词:

花花 (Chrysanthemum) 是一种花.在CmBBX8中使用.在CmFDM2中使用.这就是CmSWI3B.染色体修饰剂染色体修饰剂花开的时候,花开的时候.

更多相关视频

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Investigating Interactions Between Histone Modifying Enzymes and Transcription Factors in vivo by Fluorescence Resonance Energy Transfer

Published on: October 14, 2022

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Genome-wide Snapshot of Chromatin Regulators and States in Xenopus Embryos by ChIP-Seq

Published on: February 26, 2015

相关概念视频

Master Transcription Regulators

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...

Inheritance of Chromatin Structures

Inheritance of Chromatin Structures

Epigenetics is the study of inherited changes in a cell's phenotype without changing the DNA sequences. It provides a form of memory for the differential gene expression pattern to maintain cell lineage, position-effect variegation, dosage compensation, and maintenance of chromatin structures such as telomeres and centromeres. For example, the structure and location of the centromere on chromosomes are epigenetically inherited. Its functionality is not dictated or ensured by the underlying...

Biological Clocks and Seasonal Responses

Biological Clocks and Seasonal Responses

The circadian—or biological—clock is an intrinsic, timekeeping, molecular mechanism that allows plants to coordinate physiological activities over 24-hour cycles called circadian rhythms. Photoperiodism is a collective term for the biological responses of plants to variations in the relative lengths of dark and light periods. The period of light-exposure is called the photoperiod.

Morphogenesis

Morphogenesis

Plant morphogenesis—the development of a plant’s form and structure—involves several overlapping developmental processes, including growth and cell differentiation. Precursor cells differentiate into specific cell types, which are organized into the tissues and organ systems that make up the functional plant.

M-Cdk Drives Transition Into Mitosis

M-Cdk Drives Transition Into Mitosis

Checkpoints throughout the cell cycle serve as safeguards and gatekeepers, allowing the cell cycle to progress in favorable conditions and slow or halt it in problematic ones. This regulation is known as the cell cycle control system.
Cyclin-dependent kinases, or Cdks, work in concert with cyclins to control cell cycle transitions. M-Cdk, a complex of Cdk1 bound to M cyclin, is a well-known example of this coordinated control that drives the transition from the G2 to the M phase.
M cyclin...

Heterochromatin

Heterochromatin

The extent of chromatin compaction can be studied by staining chromatin using specific DNA binding dyes. Under the microscope, the dense-compacted regions that take up more dye are called heterochromatin. Heterochromatin is further classified into two forms – constitutive heterochromatin and facultative heterochromatin.
Constitutive heterochromatin: It is a highly compact region of chromatin that is mostly concentrated in the centromere and telomere. Unlike euchromatin, the amino acid at...