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CaNAC76通过调节CaCAD1来增强胡中的红素含量和耐寒性

Jiachang Xiao¹, Xiyu Sui¹, Zeping Xu¹

¹College of Horticulture, Sichuan Agricultural University, Chengdu 611130, China.

International journal of biological macromolecules

|December 4, 2024

概括

胡NAC转录因子CaNAC76通过增加抗氧化酶和素生产来增强耐寒性. 这通过已知的和新的途径提高了胡作物对低温的抵抗力.

科学领域:

植物科学植物科学
分子生物学分子生物学
农业科学农业科学

背景情况:

低温严重影响胡的生长,产量和产业发展.
已知NAC转录因子对寒冷压力有反应,但它们在胡中的具体作用在很大程度上是未知的.

研究的目的:

在胡 (Capsicum annuum L.) 中隔离和描述一种新的冷诱导的NAC转录因子CaNAC76.
阐明CaNAC76在植物中赋予寒冷耐受性的分子机制.

主要方法:

隔离和表征CaNAC76,包括亚细胞局部化和转录激活试验.
胡和阿拉比多普西斯中的基因沉默和过度表达,以评估耐寒性和抗氧化酶活性.
对寒冷应激反应基因表达 (ICE-CBF-COR通路) 和CaCAD1促进体结合的分析.
测量Cinnamyl酒精脱酶 (CAD) 活性和素含量.

主要成果:

CaNAC76是一种冷诱导的转录因子,定位在核和细胞质中,具有激活潜力.
沉默CaNAC76降低了胡的抗氧化酶活性和耐寒性.
在阿拉比多普西斯中过度表达CaNAC76增强了抗氧化酶活性,调高了ICE-CBF-COR基因,并改善了冷耐受性.
CaNAC76直接诱导了CaCAD1的表达,这也提高了Arabidopsis的冷耐受性.
过度表达CaNAC76增加了CAD活性和红素沉积,有助于增强结耐受性.

结论:

CaNAC76在提高胡耐寒性方面发挥着至关重要的作用.
CaNAC76通过CBF-依赖 (ICE-CBF-COR) 和CBF-独立 (CaCAD1-介导的素合成) 途径赋予寒冷耐受性.
在胡繁殖计划中,CaNAC76代表了提高抗寒应激性能的潜在目标.

关键词:

在CaCAD1中,这就是CaNAC7676的含量.寒冷的寒冷的寒冷的寒冷的寒冷的寒冷在Lignin的基础上.胡胡胡是一个

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