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从Acalypha hispida叶子中合成纳米纤维素,通过酶性水解

M P Poornima¹, Anu Anie Mathews², P Dileep³

¹Department of Chemistry, St. Joseph's College, Irinjalakuda, Thrissur, Calicut University, Kerala 680 121, India; Department of Chemistry, Sree Sankara Vidyapeetom College, Valayanchirangara, Ernakulam, Kerala 683 556, India.

International journal of biological macromolecules

|December 4, 2024

概括

使用Bacillus pumilus xylanase的Acalypha hispida叶子的酶水解有效地产生生物相容的纳米纤维素. 这种可持续的方法可以产生高质量的酶化纳米纤维素 (EHNC),用于先进的材料应用.

科学领域:

生物技术是生物技术.
材料科学材料科学材料科学
绿色化学绿色化学

背景情况:

通过酶水解合成纳米纤维素的合成还没有得到充分的研究.
叶子Acalyphahispida提供生物相容和环保的纳米纤维素的潜力.
酶方法为传统纳米纤维素生产提供了一个可持续的替代方案.

研究的目的:

为了探索Acalypha hispida叶子的酶水解,用于纳米纤维素合成.
为此过程,从菌 pumilus 中分离和描述一种新型的 ksylanase.
评估合成的酶化纳米纤维素 (EHNC) 的特性.

主要方法:

使用16S rRNA测序分离和识别菌.
通过硫酸沉和透析净化西兰酶.
使用纯化西兰酶合成纳米纤维素,并对EHNC特性 (颗粒大小,泽塔潜力) 的表征.

主要成果:

鉴定出一种产生西兰酶的Bacillus pumilus菌株,并对其酶进行净化 (45kDa).
酶化纳米纤维素 (EHNC) 从Acalypha hispida叶子中成功合成.
EHNC的粒子直径为15-40nm,泽塔电位为-22.3mV,表明稳定性.

结论:

使用Bacillus pumilus xylanase的酶水解是一种可行的和可持续的方法来生产EHNC.
该过程是高效的,需要最小的酶度和短的潜伏时间.
这种方法为制造基于纳米纤维素的先进材料提供了一条绿色和成本效益的途径.

关键词:

阿卡利法希斯皮达希斯皮达菌菌 (Bacillus pumilus) 是一种菌.细菌分离的细菌分离方法纤维素纳米纤维纤维素纳米纤维酶水解是酶水解的方法之一.在Xylanase的使用中.

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