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低温应激对抗寒性物质和类 (Lepidoptera: Noctuidae) 的基因表达的影响
Huawei Ren1, Junrui Zhi1, Dingyin Li1
1Institute of Entomology, Guizhou University/Guizhou Provincial Key Laboratory for Agricultural Pest Management of the Mountainous Region, Guizhou, Guiyang, 550025, China.
Cryobiology
|December 4, 2024
概括
秋季军 (Spodoptera frugiperda) 幼虫在冬季积累脂质,三糖和糖醇,以提高耐寒性. 抑制脂酶基因 (Eol1,Epl1) 降低了生存率,这表明脂质对抗寒性至关重要.
科学领域:
- 农业昆虫学 农业昆虫学
- 昆虫生理学 昆虫生理学
- 分子生物学分子生物学
背景情况:
- 秋季军虫 (Spodoptera frugiperda) 是一个重要的农业害虫,影响着全球的作物产量.
- 低温极大地影响S. frugiperda的生存和繁殖能力,需要了解其耐寒机制.
- 昆虫生理学的季节性变化是了解害虫过冬策略和制定有效控制措施的关键.
研究的目的:
- 在冬季月份 (2022年10月至2023年2月) 调查Spodoptera frugiperda幼虫的生理寒冷耐受性指数.
- 评估特定基因,内皮脂酶 (Eol1) 和胃脂酶 (Epl1) 在S. frugiperda的耐寒性中的作用.
- 为了确定脂质对秋季军幼虫耐寒性的贡献.
主要方法:
- 在不同月份和幼虫阶段的S. frugiperda幼虫中量化耐寒物质 (水,脂质,三糖,蛋白质,糖醇).
- 转录组分析以确定参与抗寒的候选基因,重点是Eol1和Epl1.
- 通过RNA干扰 (RNAi) 沉默Eol1和Epl1基因,然后评估基因表达,存活率,脂质含量和在寒冷压力下幼体重.
主要成果:
- 耐寒物质含量因月份而异,含水量最低的是1月,脂质含量最高的是12月和1月.
- 脂质,三糖和糖醇含量在第六阶段幼虫中达到峰值,这表明发育阶段影响了寒冷耐受性.
- 沉默Eol1和Epl1显著降低了它们的mRNA水平,降低了幼虫存活率,降低了脂质含量,并在寒冷压力下降了幼体重.
结论:
- 幼虫表现出生理适应,包括改变的代谢物含量,以生存寒冷的冬季条件.
- 脂质在秋季军幼虫的抗寒性中发挥着至关重要的作用.
- 基因Eol1和Epl1对于维持脂质水平和S. frugiperda在寒冷压力下整体生存非常重要.
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