使用无标签零电压光电化学生物传感器对PAE总量的全面和敏感分析
Xin Zheng1, Yajing Ji2, Shengjie Li3
1Department of Laboratory Medicine, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China.
ACS applied materials & interfaces
|December 4, 2024
概括
一个新的光电化学 (PEC) 生物传感器有效地检测复杂样品中的甲酸 (PAE). 这种敏感的方法为环境监测和公共卫生保护提供了一个有前途的工具.
科学领域:
- 环境科学 环境科学
- 分析化学 分析化学
- 生物技术是生物技术.
背景情况:
- 酸盐 (PAE) 对人类健康和环境构成风险.
- 在复杂矩阵中准确检测PAE是具有挑战性的.
- 开发敏感和选择性的分析方法至关重要.
研究的目的:
- 开发一种高度敏感和选择性的光电化学 (PEC) 生物传感器,用于PAE分析.
- 使用一种新的生物传感器设计,以改进PAE检测.
- 在真实世界样本中验证生物传感器的性能.
主要方法:
- 一个CdS纳米粒子/TiO2纳米管 (CdS NP/TiO2 NT) 电极的制造.
- 为特定的PAE识别,截断的PAE适合体 (PAEs-apt) 的固定.
- 光电化学检测利用界面电阻变化.
主要成果:
- 生物传感器对PAE的灵敏度高于SELEX亚胺相比.
- 线性检测范围从0.005到1 ng/mL,低检测极限为1.67 ng/L.
- 优秀的选择性,误差系数<0.277,即使是100倍的干扰物种度.
结论:
- 开发的CdS NP/TiO2 NT/PAE适合的PEC生物传感器为PAE检测提供了一种高度敏感和选择性的方法.
- 该生物传感器有效地分析PAE在尿液和水等复杂样品中的PAE.
- 这种方法提供了一个简单而敏感的分析工具,用于检测PAE和类似化合物.
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