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实时在活体中测试人类皮肤使用手持的富里埃变换红外光谱仪,采用三跳两通减弱总反射接口

Samuel F Williams¹, John Chittock¹, Kirsty Brown¹

¹Sheffield Dermatology Research, Department of Infection, Immunity and Cardiovascular Disease, University of Sheffield Medical School, Sheffield, UK.

Applied spectroscopy

|December 5, 2024

概括

一个新的三跳两通 (3B2P) 减弱总反射 (ATR) 里叶变换红外光谱 (FT-IR) 接口显著提高了皮肤分析的便携式光谱仪性能. 这种新型的接口可以提高皮肤病应用中的灵敏度和数据质量.

科学领域:

频谱学是一种光谱学.
生物医学工程生物医学工程
皮肤病学皮肤病学

背景情况:

减弱总反射 (ATR) 里叶变换红外光谱法 (FT-IR) 是一种多功能分子表征技术.
便携式FT-IR光谱仪提供现场分析,但在灵敏度和数据质量方面可能存在局限性.
标准的一跳 (1B) ATR接口在便携式设备中很常见.

研究的目的:

在便携式光谱仪上比较新型三跳两通 (3B2P) ATR接口与标准一跳 (1B) ATR接口的性能.
为了评估这些接口与台式10反弹 (10B) ATR光谱仪对比.
在皮肤学研究中评估这些ATRFT-IR配置的实用性,特别是用于新生儿皮肤分析.

主要方法:

在便携式光谱仪上的1B和3B2PATR接口与10BATR台面光谱仪的性能比较.
在理想条件下对异醇的灵敏度和信号噪声比 (SNR) 的分析.
在一项涉及180名健康新生儿的皮肤学研究中应用,以评估高质量的频谱采集率和出生和四周SNR.

主要成果:

基板10B ATR显示了最高的SNR,但新的3B2P接口使便携式光谱仪对异醇的灵敏度增加了六倍.
与1B接口相比,3B2P接口对异醇和体内皮肤样本的SNR一致性更高.
在皮肤学研究中,3B2P接口在两个时间点都比1B接口取得了55%更高的高质量频谱收集率,并且比1B接口更好的SNR.

结论:

新的3B2P ATR接口显著提高了皮肤病应用的便携式FT-IR光谱仪的性能.
这项技术在临床应用中显示出前途,使得可以量化皮肤特性,例如新生儿的水分水平.
特别是具有3B2P等先进接口的ATR FT-IR,具有识别皮肤疾病生物标志物的潜力.

关键词:

在ATR的FT-IR中,临床临床临床临床在这里,我们可以看到 IR IR IR IR.减弱的总反射里埃变换红外光谱学在红外线下,红外线是指红外线.

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