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Updated: Jun 5, 2025

Pooled CRISPR-Based Genetic Screens in Mammalian Cells
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Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Published on: September 4, 2019

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编排的CRISPR库用于全基因组激活,删除和沉默人类蛋白质编码基因

Jiang-An Yin1, Lukas Frick2, Manuel C Scheidmann3

  • 1Institute of Neuropathology, University of Zurich, Zurich, Switzerland. jiang-an.yin@uzh.ch.

Nature biomedical engineering
|December 5, 2024
PubMed
概括

研究人员开发了新的四倍单指导RNA (sgRNA) 库,用于高效的全基因组基因扰动屏幕. 这些图书馆能够进行强大的基因切除,激活和沉默,推进功能基因组学研究.

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CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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