在卵细胞冷保存后连续八年临床利用和结果
Gayathree Murugappan1, Mark Sikder2, Sergio Vaccari2
1Spring Fertility, San Francisco, CA, USA. gaya.murugappan@springfertility.com.
Journal of assisted reproduction and genetics
|December 5, 2024
概括
卵细胞冷保存 (OC) 与新鲜卵细胞相比显示出较低的胚胎细胞发育,特别是在老年人中. 然而,在从新鲜和解的卵子细胞中转移欧普洛伊德胚胎细胞后,临床怀孕和活产率仍然相似.
科学领域:
- 生殖科学 生殖科学
- 胚胎学 胚胎学
- 低温生物学 低温生物学
背景情况:
- 卵细胞冷保存 (OC) 是一种成熟的生育保存技术.
- 将OC与即时胚胎冷保存 (EC) 的结果进行比较对于优化生育治疗至关重要.
研究的目的:
- 为了比较胚胎和临床结果之间的卵细胞冷保存 (OC) 和立即受精胚胎冷保存 (EC) 在非不孕的患者.
- 评估卵细胞年龄对解后胚胎细胞发育的影响.
主要方法:
- 从2016年到2023年对非不育患者 (年龄在18-42岁) 的回顾性队列研究.
- 对3697个解卵子 (OC组) 和28959个新鲜卵子 (即时EC组) 的结果进行比较.
- 采用了细胞内质精子注射 (ICSI),移植前遗传测试 (PGT-A) 和单个胚胎移植 (eSET).
主要成果:
- 与新鲜卵子 (90%) 相比,解卵子的受精率较低 (85%).
- 胚胎细胞的发育效率低于解卵细胞,随着年龄的增长,每个胚胎细胞的MII卵细胞需求增加.
- 在解和新鲜卵细胞组之间,临床怀孕和活产率每次欧卵母细胞移植均相似.
结论:
- 与新鲜卵子相比,解卵子的胚胎细胞转化率较低.
- 胚胎细胞发育中的效率差距随着卵子细胞年龄的增加而扩大,后玻璃化和变暖.
- 虽然胚胎潜力不同,但OC仍然是一个可行的选择,在转移欧卵母细胞时,其临床结果与即时EC相似.
相关概念视频
02:07Oogenesis
63.4K
In human women, oogenesis produces one mature egg cell or ovum for every precursor cell that enters meiosis. This process differs in two unique ways from the equivalent procedure of spermatogenesis in males. First, meiotic divisions during oogenesis are asymmetric, meaning that a large oocyte (containing most of the cytoplasm) and minor polar body are produced as a result of meiosis I, and again following meiosis II. Since only oocytes will go on to form embryos if fertilized, this unequal...
63.4K
01:57Meiosis II
182.8K
Meiosis II is the second and final stage of meiosis. It relies on the haploid cells produced during meiosis I, each of which contain only 23 chromosomes—one from each homologous initial pair. Importantly, each chromosome in these cells is composed of two joined copies, and when these cells enter meiosis II, the goal is to separate such sister chromatids using the same microtubule-based network employed in other division processes. The result of meiosis II is two haploid cells, each...
182.8K