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单细胞RNA测序分析揭示了肝细胞癌中潜在的关键预后标志物

Heteng Cui¹, Wenyuan Yang²

¹Department of Oncology, The 940Th Hospital of Joint Logistic Support Force of Chinese People's Liberation Army, 333 Nan Bin He Road, Qilihe District, Lanzhou, 730050, Gansu, China.

Discover oncology

|December 5, 2024

概括

这项研究确定了使用单细胞RNA测序来预测肝细胞癌 (HCC) 患者存活率的关键基因. 这些发现为改善HCC患者的治疗结果提供了潜在的预后标志物.

科学领域:

在瘤学瘤学.
基因组学就是基因组学.
生物信息学是一种生物信息学.

背景情况:

肝细胞癌 (HCC) 是一种流行癌症,存活率较低.
识别预后性遗传标记对于HCC患者管理至关重要.

研究的目的:

用单细胞RNA测序 (scRNA-seq) 分析预测肝细胞癌 (HCC) 预后的遗传特征.
识别和验证HCC.中的新型预后标记基因.

主要方法:

从基因表达综合 (GEO) 数据库中分析scRNA-seq数据.
利用Seurat进行质量控制,正常化和细胞聚类.
进行了功能丰富 (GO,KEGG),细胞间通信,轨迹和LASSO-Cox回归分析.
通过RT-qPCR和西欧斑块验证的基因表达和预后意义.

主要成果:

在HCC样本中确定了2208个上调和1447个下调基因.
发现了566个预后基因,其中11个通过LASSO-COX回归选择.
五个基因被确定为不良预后标记物,并在HCC细胞系中过度表达.

结论:

该研究确定了HCC患者潜在的预后标记基因.
这些发现为预测HCC患者生存率提供了洞察力.

关键词:

肝细胞癌是肝细胞癌.预后标志物是一个预后标志物.信号通道的信号通道.单细胞RNA测序的一个细胞.幸存的生存方式

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lncRNA - Long Non-coding RNAs

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...