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相关概念视频

Gastrulation01:56

Gastrulation

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Gastrulation establishes the three primary tissues of an embryo: the ectoderm, mesoderm, and endoderm. This developmental process relies on a series of intricate cellular movements, which in humans transforms a flat, “bilaminar disc” composed of two cell sheets into a three-tiered structure. In the resulting embryo, the endoderm serves as the bottom layer, and stacked directly above it is the intermediate mesoderm, and then the uppermost ectoderm. Respectively, these tissue strata...
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Cleavage and Blastulation01:33

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After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
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Determination01:51

Determination

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During embryogenesis, cells become progressively committed to different fates through a two-step process: specification followed by determination. Specification is demonstrated by removing a segment of an early embryo, “neutrally” culturing the tissue in vitro—for example, in a petri dish with simple medium—and then observing the derivatives. If the cultured region gives rise to cell types that it would normally generate in the embryo, this means that it is specified. In...
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相关实验视频

Updated: Jun 5, 2025

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
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Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

Published on: January 26, 2013

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Bmlark对于胚胎发育至关重要.

Yuling Peng1,2, Jin Li1, Kangkang Niu1

  • 1Guangdong Key Laboratory of Insect Developmental Biology and Applied Technology, Guangzhou Key Laboratory of Insect Development Regulation and Application Research, Institute of Insect Science and Technology, School of Life Sciences, South China Normal University, Guangzhou, 510631, China.

BMC genomics
|December 5, 2024
PubMed
概括
此摘要是机器生成的。

转录因子Bmlark对于丝虫胚胎发育至关重要. 失去Bmlark功能会导致致命的发育缺陷,影响皮质的形成和黑色化.

关键词:
在Bmlark上,我们可以看到波姆比克斯 (Bombyx mori mori mori) 是一个有趣的动物.胚胎发育的时间梅拉尼化的过程一个突变的突变者.硬化成硬化形成的情况.

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A Rapid In Vivo Bioassay for Developmentally Active Enhancers

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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

Published on: January 12, 2022

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相关实验视频

Last Updated: Jun 5, 2025

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development
14:08

Blastomere Explants to Test for Cell Fate Commitment During Embryonic Development

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A Rapid In Vivo Bioassay for Developmentally Active Enhancers
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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay
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Analysis of Cell Differentiation, Morphogenesis, and Patterning During Chicken Embryogenesis Using the Soaked-Bead Assay

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科学领域:

  • 发展生物学 发展生物学
  • 遗传学 是一个遗传学.
  • 分子生物学分子生物学

背景情况:

  • 已知Drosophila的转录因子的功能,但其在Bombyx mori胚胎发育中的作用需要阐明.
  • 研究在丝虫中的功能可以揭示保存的发育机制.

研究的目的:

  • 调查转录因子Bmlark在Bombyx mori胚胎发育中的功能.
  • 在丝虫胚胎发生过程中识别由Bmlark调节的基因.

主要方法:

  • 使用CRISPR/Cas9基因编辑创建了一个Bmlark突变Bombyx mori菌株.
  • 进行了转录组分析 (RNA-Seq),以比较Bmlark突变和野生类型胚胎之间的基因表达.

主要成果:

  • 同卵性Bmlark突变胚胎 (Bmlark-/-) 是致命的,表现出外骨硬化和黑化缺陷.
  • 转录组分析显示,Bmlark-/-胚胎中63个假定皮质蛋白基因和5个色素合成基因的表达有显著的改变.
  • 关键的基因参与皮层形成和氨酸介导色素化途径显示差异性表达,与下调大多数色素合成基因.

结论:

  • Bmlark对于正常的丝虫胚胎发育至关重要,特别是在皮层形成和黑色化方面.
  • Bmlark调节了许多皮质蛋白基因和参与色素合成的基因的表达.