洞察了在人类性压力下的Hevea brasiliensis中 lncRNA介导的调节网络
Yanluo Zeng1, Tianbin Guo1, Liping Feng2
1School of Tropical Agriculture and Forestry, Hainan University, Haikou, Hainan, China.
Plant methods
|December 5, 2024
概括
这项研究揭示了非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 调节树 (Hevea brasiliensis) 中长时间的反菌耐药性的程度. 了解这些非编码RNA为改善作物保护提供了新的策略.
科学领域:
- 植物分子生物学 植物分子生物学
- 基因组学就是基因组学.
- 农业科学 农业科学
背景情况:
- 长非编码RNAs (lncRNAs) 和microRNAs (miRNAs) 是植物基因网络中的关键调节者.
- 炭病对树 (Hevea brasiliensis) 种植构成重大威胁,影响乳产量和质量.
- 在Hevea brasiliensis对炭糖的反应中,lncRNAs和miRNAs的确切作用仍然在很大程度上未被探索.
研究的目的:
- 为了研究在人类毒性压力下的Hevea brasiliensis中lncRNAs和miRNAs的调节作用.
- 确定关键的非编码RNA及其参与疾病耐药性的调节网络.
- 为了揭示增强树抗甲基耐药性的机制.
主要方法:
- 对lncRNA测序,miRNA测序和降解组测序数据的综合分析.
- 差异表达 lncRNAs (DE-lncRNAs) 的识别和表征.
- 构建一个具有竞争力的内源RNA (ceRNA) 监管网络.
主要成果:
- 解读了在人类鼻感染期间Hevea brasiliensis中lncRNAs和miRNAs的调节格局.
- 确定了特定的调节通路,包括HblncRNA29219/hbr-miR482a和miR390-TAS3-ARF通路,对抗性至关重要.
- 阐明了这些非编码RNA在增强甲基耐药性的功能作用.
结论:
- 非编码RNA在调解Hevea brasiliensis对真菌病原体的反应中起着至关重要的作用.
- 鉴定到的lncRNA-miRNA相互作用为改善树抗病能力提供了新的标.
- 这些发现有助于理解植物微生物相互作用,并制定可持续的农业战略.
相关概念视频
02:39lncRNA - Long Non-coding RNAs
8.5K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.5K
01:31Regulation of the Unfolded Protein Response
2.4K
Inositol-requiring kinase one or IRE1 is the most conserved eukaryotic unfolded protein response (UPR) receptor. It is a type I transmembrane protein kinase receptor with a distinctive site-specific RNase activity. As the binding mechanics of the misfolded proteins with the N-terminal domain of IRE-1 are unclear, three binding models — direct, indirect, and allosteric -- are proposed for receptor activation. Nevertheless, it is known that once a misfolded protein associates with IRE1, it...
2.4K
01:27Constitutive and Regulated Gene Expression
Gene expression in prokaryotes is governed by constitutive and regulated systems, allowing cells to balance the production of essential proteins with adaptive responses to environmental changes.Constitutive Gene ExpressionConstitutive, or housekeeping, genes are continuously expressed as they encode proteins vital for fundamental cellular processes. These include enzymes for glycolysis, ribosomal components for protein synthesis, and proteins involved in DNA replication. Their constant...
01:25Cell Signaling in Plants
5.6K
Plant cells communicate to coordinate their cycle of growth, flowering and fruiting, and activities in roots, shoots, and leaves in response to the changing environmental conditions. Plant signaling is distinct from animal signaling. Plants primarily utilize enzyme-linked receptors, whereas the largest class of cell-surface receptors in animals are G-protein coupled receptors (GPCRs). Unlike animals, receptor tyrosine kinases are rare in plants. Instead, plants have a diverse class of...
5.6K
02:24Regulation of Expression Occurs at Multiple Steps
22.4K
Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...
22.4K
01:23Regulation of Expression at Multiple Steps
869
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
869