Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

6.9K
Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been...
6.9K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Severe trauma care: advances and future directions in diagnostic and therapeutic techniques and information technology support.

Medical review (2021)·2026
Same author

Sensitivity suppression during attention shifts.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Clinical Prognostic Modeling and Paired Blood-CSF Metabolomic Profiling for Outcome Prediction in Isolated Moderate-to-Severe Traumatic Brain Injury: Implications for Neurocritical Care Management.

Journal of clinical medicine·2026
Same author

Unrolled Richardson-Lucy deconvolution network with partially connected layers in computational microscopy.

Optics express·2026
Same author

Lithium-Germanium Alloy Interfaces for Efficient Low-Pressure Ammonia Synthesis.

Angewandte Chemie (International ed. in English)·2026
Same author

SIRT5-RNF126 coordinated regulation of METTL17 stability controls mitochondrial function and glioma progression.

Cell & bioscience·2026
Same journal

Adaptive angle-calibration for wide field-of-view on-chip phase imaging with LED array.

Biomedical optics (Washington, D.C.)·2026
Same journal

On-chip quantitative phase microscopy without support constraint.

Biomedical optics (Washington, D.C.)·2025
Same journal

GigaFIBI; Rapid, Large-format Histology-resolution Imaging for Intraoperative Assessment of Breast Lumpectomy Margins.

Biomedical optics (Washington, D.C.)·2025
Same journal

Simultaneous Dual-region Functional Imaging in Miniaturized Two-photon Microscopy.

Biomedical optics (Washington, D.C.)·2024
Same journal

High Speed Miniaturized Multiphoton Microscopy with Elliptical Beam Excitation.

Biomedical optics (Washington, D.C.)·2024
Same journal

Convolutional Neural Network-based Optical Coherence Tomography (OCT) A-scan Segmentation and Tracking Platform using Advanced Monte Carlo Simulation.

Biomedical optics (Washington, D.C.)·2023
查看所有相关文章

相关实验视频

Updated: Jun 5, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.4K

时间复合小型化双光子显微镜

Shing-Jiuan Liu1, Zixiao Zhang1, Ben Mattison2,3

  • 1Department of Electrical and Computer Engineering, University of California, Davis, Davis, CA 95616, USA.

Biomedical optics (Washington, D.C.)
|December 5, 2024
PubMed
概括
此摘要是机器生成的。

我们开发了一种更快,小型化的两光子显微镜 (TM-MINI2P),用于在小鼠大脑中高速的体内成像. 这一进步使成像速度翻了一番,而不会牺牲空间分辨率.

更多相关视频

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.8K
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
05:45

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

7.4K

相关实验视频

Last Updated: Jun 5, 2025

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy
10:41

Visualizing Protein Kinase A Activity In Head-fixed Behaving Mice Using In Vivo Two-photon Fluorescence Lifetime Imaging Microscopy

Published on: June 7, 2019

8.4K
A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins
16:10

A TIRF Microscopy Technique for Real-time, Simultaneous Imaging of the TCR and its Associated Signaling Proteins

Published on: March 22, 2012

23.8K
In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin
05:45

In Vivo Two-Color 2-Photon Imaging of Genetically-Tagged Reporter Cells in the Skin

Published on: July 11, 2019

7.4K

科学领域:

  • 神经科学是一个神经科学.
  • 生物医学工程 生物医学工程
  • 光学成像技术的成像

背景情况:

  • 高速体外成像对于理解神经动力学至关重要.
  • 现有的双光子显微镜技术在速度或分辨率方面面临限制.
  • 微型化是先进的in-vivo神经科学应用的关键.

研究的目的:

  • 推出一种新的时间复合小型化双光子显微镜 (TM-MINI2P).
  • 为了证明TM-MINI2P的高速,高分辨率的体外成像能力.
  • 为了验证其在小鼠皮质电路分析中的应用.

主要方法:

  • 开发一个时间复合的小型化双光子显微镜 (TM-MINI2P).
  • 实施先进的光学设计,以提高速度.
  • 在小鼠皮质中的活体成像实验.

主要成果:

  • 与传统方法相比,成像速度提高了两倍.
  • 在高速成像过程中保持高空间分辨率.
  • 成功捕获了小鼠大脑中的动态神经活动.

结论:

  • TM-MINI2P为高速体内神经成像提供了显著的进步.
  • 这项技术可以在更快的时间范围内更深入地了解大脑功能.
  • 微型化双光子显微镜是未来神经科学研究的一个有前途的工具.