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基于终身成像,在活细胞中进行四维多粒子跟踪

Danni Chen¹, Heng Li^1,2, Bin Yu¹

¹Center for Biomedical Optics and Photonics (CBOP) & College of Physics and Optoelectronic Engineering, Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, Shenzhen University, Shenzhen, 518060, China.

Nanophotonics (Berlin, Germany)

|December 5, 2024

概括

这项研究引入了一种新的四维 (4D) 多粒子并行跟踪方法,用于观察动态细胞过程. 它同时捕捉活细胞中移动分子的空间位置和局部微环境变化.

科学领域:

细胞生物学细胞生物学
生物物理学的生物物理.
显微镜的使用方法

背景情况:

了解活细胞中的动态事件,如细胞内运输,对于细胞功能至关重要.
细胞微环境可以显著影响移动囊泡和生物大分子的行为.

研究的目的:

开发一种方法,同时监测活细胞中的多个粒子的空间位置和局部环境变化 (通过光寿命).
为了实现四维 (4D) 多粒子并行跟踪,以加强细胞过程研究.

主要方法:

使用双螺旋点扩散功能 (DH-PSF) 显微镜与线条相机相结合.
集成的三维 (3D) 定位与光寿命成像通过修改点传播函数 (PSF).
开发了一个概念验证的4D跟踪系统.

主要成果:

实现的3D定位精度为σ(x,y,z) = (26nm,35nm,53nm) 和光寿命精度为σ(τ) = 103ps.
证明了有效的景深大约为4微米.
在4微米的轴深度内,在细胞内内细胞内激活过程中成功追踪了微球.

结论:

开发的4D多粒子并行跟踪方法提供了活细胞中移动目标的同时物理和化学信息.
这种技术通过揭示微环境对分子行为的影响,为研究动态细胞过程提供了新的视角.
该系统的功能通过模拟和细胞内运动的实验跟踪来验证.

关键词:

光终身显微镜显微镜多颗粒追踪系统的多颗粒追踪系统单颗粒追踪器是一个单颗粒追踪器.

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