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肠道微生物群通过Gαq/11介导的STAT1/4途径诱导巨细胞中的CLA和IL-35诱导:一项基于动物的研究

Xiaomin Su¹, Yazheng Yang¹, Yunhuan Gao¹

¹Department of Immunology, Nankai University School of Medicine, Nankai University, Tianjin, China.

|December 5, 2024

概括

再生蛋白4 (REG4) 通过影响肠道微生物群的组成来维持肠道免疫平衡. 这种蛋白质促进了烯酸转化为联烯酸,从而促进了巨细胞中IL-35的表达.

科学领域:

免疫学免疫学免疫学
微生物学微生物学
胃肠病学胃肠病学

背景情况:

肠道微生物群和代谢物对宿主新陈代谢,免疫反应和恒温至关重要.
肠道微生物群的变化与肠道和肠外疾病有关,例如炎症性肠道疾病 (IBD).
肠上皮细胞产生调节肠道微生物群的杀菌物质,但再生蛋白4 (REG4) 的作用尚不清楚.

研究的目的:

研究REG4在维持肠道免疫平衡中的作用.
阐明 REG4 影响肠道微生物群和免疫反应的机制.

主要方法:

使用了Reg4淘汰赛 (KO) 小鼠和人类REG4肠上皮细胞转基因 (huREG4) 小鼠.
采用DSS介导性结肠炎模型来评估疾病的敏感性.
通过测序分析了肠道微生物组成.
使用液体染色学质谱学量化的代谢物水平.
通过免疫沉和免疫阻塞来研究蛋白质表达.

主要成果:

Reg4 KO小鼠对DSS诱导的大肠炎的敏感性增加,而huREG4小鼠更具耐药性.
REG4/Reg4显著改变了肠道微生物群的组成,包括促进*乳杆菌*物种.
*乳杆菌*将酸 (LA) 代谢成联合酸 (CLA). *乳杆菌*将酸代谢成联合酸 (CLA).
通过Gαq/11介导的STAT1/4激活,CLA增强了巨细胞中的IL-35表达.

结论:

REG4对于通过其关联的肠道微生物群来维持肠道免疫平衡至关重要.
该机制涉及REG4介导的微生物群调节以产生CLA,然后诱导巨细胞中的IL-35表达.
REG4在通过CLA-IL-35巨轴调节肠道免疫力方面发挥着至关重要的作用.

关键词:

结合的烯酸是合的.它们的IL-35是IL-35.在 Reg4 中,您可以在 Reg4 中使用 Reg4 文件.我们的肠道微生物群.巨细胞是什么?巨细胞是什么?

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