在实验性腹膜透析中,F-脱氧葡萄糖通过促进性葡萄糖通道进行细胞间运输
Giedre Martus1, Premkumar Siddhuraj2, Jonas S Erjefält2,3
1Nephrology Division, Department of Clinical Sciences, Skåne University Hospital, Lund University, Lund, Sweden.
概括
葡萄糖运输体 (GLUT) 在腹腔透析 (PD) 流体运输中发挥作用. 用弗洛雷丁抑制GLUT改善了超过,并建议通过腹膜传输细胞间葡萄糖.
科学领域:
- 腹膜透析研究 腹膜透析研究
- 膜运输机制是膜运输机制.
- 生物医学工程 生物医学工程
背景情况:
- 腹腔透析 (PD) 中的葡萄糖运输限制会影响治疗的有效性.
- 罗,一种葡萄糖转运体 (GLUT) 抑制剂,降低了葡萄糖扩散,表明GLUT参与PD.
- 葡萄糖通过腹膜细胞的运输路径 (吸收与跨细胞) 仍然不清楚.
研究的目的:
- 研究GLUTs在PD期间跨细胞葡萄糖运输中的作用.
- 为了确定GLUT抑制是否影响从血到透析物中的葡萄糖扩散.
- 要区分吸收和跨细胞运输假设.
主要方法:
- 使用放射性标记的18F-脱氧葡萄糖 ([18F]-DG) 来追踪葡萄糖的运输.
- 施用了佛洛来抑制GLUTs,并评估了它对[18F]-DG扩散的影响.
- 监测其他溶液的运输,以区分半细胞和跨细胞通路.
主要成果:
- 用弗洛瑞丁抑制GLUT显著降低了[18F]-DG扩散能力.
- 超过率 (UF) 得到改善,在GLUT抑制下,度下降得到增强.
- 尿素和二碳酸盐的运输基本上不受影响,这支持GLUT在葡萄糖运输中的特定作用.
结论:
- 葡萄糖部分通过跨细胞路径穿过腹膜进行运输.
- 促进性GLUTs的抑制剂显示出在PD患者中增强UF疗效的潜力.
- 准GLUT可能为改善PD治疗结果提供一种新的策略.
关键词:
BAY-87676 航空公司这就是 GLUT1 的原因.腹膜透析是指腹膜透析.[18F] - 脱氧葡萄糖 - 脱氧葡萄糖印度纳维尔 (indinavir) 是一种药物.佛罗里丁是一种色素.里托纳维尔 (ritonavir) 是一种药物.超过的效率是超过的效率.更多相关视频
11:13Quantifying Glomerular Permeability of Fluorescent Macromolecules Using 2-Photon Microscopy in Munich Wistar Rats
Published on: April 17, 2013
10.4K
09:40Isolation, Characterization, And High Throughput Extracellular Flux Analysis of Mouse Primary Renal Tubular Epithelial Cells
Published on: June 20, 2018
17.4K
相关概念视频
Transcellular Transport of Solutes
3.4K
Transcellular transport of solutes is the movement of substances like monosaccharides and amino acids through polarized cells. This transport mechanism is primarily seen in epithelial and endothelial cells aided by membrane transport proteins such as channels and transporters. The tight junctions between these cells confine the membrane proteins to the two sides of the cell. The epithelial cells have distinct apical and basolateral domains. In contrast, the endothelial cells show the luminal...
3.4K
Glucose Absorption Into the Small Intestine
31.3K
Complex carbohydrates consumed cannot be absorbed into the small intestine in their original form. First, they must be hydrolyzed to a monosaccharide form such as glucose or galactose. These monosaccharides are then transported across the intestinal membrane and into the blood via transcellular transport. The intestinal epithelial cells allow the movement of these monosaccharides with a defined 'entry' through membrane transporter proteins present on their apical membrane and...
31.3K
Secondary Active Transport
6.8K
One example of how cells use the energy contained in electrochemical gradients is demonstrated by glucose transport into cells. The ion vital to this process is sodium (Na+), which is typically present in higher concentrations extracellularly than in the cytosol. Such a concentration difference is due, in part, to the action of an enzyme "pump" embedded in the cellular membrane that actively expels Na+ from a cell. Importantly, as this pump contributes to the high concentration of...
6.8K
Facilitated Diffusion
310
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
310
Dialysis
589
Dialysis is a diffusion-based purification process that separates analyte molecules from a complex matrix. This is accomplished by allowing molecules in the solution to pass through a semipermeable membrane into a liquid on the other side. The membrane is usually made of cellulose acetate or cellulose nitrate, and the second liquid must be miscible with the solution. Ions (e.g., chloride or sodium) or organic molecules (e.g., glucose) can pass through the membrane pores, which generally have...
589
Glucose Transporters
22.5K
Glucose transporters facilitate the transport of glucose across the cell membrane. In addition to glucose, some glucose transporters can also aid the movement of other hexoses such as fructose, mannose, and galactose.
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
Facilitated diffusion-glucose transporters (GLUTs) are encoded by the solute-linked carrier (SLC) family 2, subfamily A gene family, or SLC2A. The 14 GLUT protein members are distributed into three classes:
22.5K
