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Updated: Jun 5, 2025

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针对大型基因组片段的直接克隆策略:一篇综述

Ya-Nan Chen1, You-Zhi Cui1, Xiang-Rong Chen1

  • 1Frontiers Science Center for Synthetic Biology and Key Laboratory of Systems Bioengineering (Ministry of Education), School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China; Frontiers Research Institute for Synthetic Biology, Tianjin University, Tianjin 30072, China.

Biotechnology advances
|December 5, 2024
PubMed
概括

直接克隆大型DNA片段 (50-150 kb) 是合成生物学的进步. 本综述强调了最近的克隆技术及其在功能基因组学和自然产品发现中的应用.

关键词:
生物医学是生物医学.生物技术是生物技术.直接克隆就是直接克隆.大量的基因组碎片.自然产品是一种自然产品.合成生物学 合成生物学

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DNA isolation protocols can be fast and straightforward or complex and time-consuming depending on the type and quality of DNA required for further processing. For example, plasmid DNA extraction is a bit more complicated than genomic DNA extraction because of the need for an appropriate lysis method to separate plasmid DNA from gDNA during isolation. However, for specific applications, such as long-range DNA sequencing that require a good yield of high- quality DNA samples, we need to follow...
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