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利用透冲击来加强 (纳米) 货物的细胞内输送

Beatrice Ruzzante¹, Flaminia Fruzzetti¹, Marco Cattaneo²

¹genT_LΛB, Dept. of Chemistry, Materials and Chemical Engineering "Giulio Natta", Politecnico di Milano, Milan, Italy.

International journal of pharmaceutics

|December 5, 2024

概括

低位性透冲击暂时改变细胞形状而不会造成伤害,显著提高纳米粒子和治疗载荷的吸收. 这种方法绕过了传统的内细胞结合,为细胞内输送提供了一种新的方法.

科学领域:

细胞生物学细胞生物学
纳米技术纳米技术
药物运输药物运输药物运输

背景情况:

纳米材料的有效细胞内传递对研究和治疗至关重要.
货物的物理化学性质对内部化效率有很大的影响.
优化纳米交付方法是一个活跃的研究领域.

研究的目的:

作为一种增强纳米粒子和治疗载荷内部化的方法来研究透冲击.
评估低压和高压冲击对细胞活力,体积,膜张力和颗粒吸收的影响.
探索奥斯莫斯冲击诱导的内化背后的机制.

主要方法:

向初级和细胞系施加了低压和高压的透冲击.
监测细胞活力,体积和膜张力.
在不同的透条件下量化颗粒吸收 (100-nm和500-nm).
研究了内部化途径,包括内细胞化.
在肌缩侧面硬化症 (ALS) 患者的纤维细胞中测试了治疗性货物输送.

主要成果:

短期的透冲击并没有影响细胞活力.
低压冲击诱导了细胞形状的短暂变化 (5分钟),恢复时间为15分钟.
低压冲击使100nm和500nm粒子吸收分别增加了约3倍和5倍.
超性休克对细胞行为或粒子吸收没有显著影响.
透冲击介导的吸收独立于活跃的内细胞通路.
低压冲击增强了治疗载荷 (icerguastat) 输送和ALS纤维细胞中的miR-106b-5p表达.

结论:

透冲击,特别是低压冲击,是一种安全有效的策略,可以增强纳米粒子和治疗剂的细胞内传递.
这种方法对难以治疗的细胞有益,并为组织中局部药物输送提供了潜在的潜力.
非内细胞机制扩大了改善药物递送效率的选择.

关键词:

细胞刺激细胞刺激细胞吸收的过程细胞体积细胞体积细胞体积药物交付是药物交付的过程.纳米颗粒的交付方式透冲击是一种冲击.

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