来自Bifidobacterium longum亚种的外聚糖. 婴儿和青少年的Bifidobacterium调节托尔类受体信号传递
Renate Akkerman1, Marjolein M P Oerlemans1, Michela Ferrari2
1Department of Pathology and Medical Biology, University Medical Center Groningen, Groningen, The Netherlands.
来自Bifidobacterium细菌的外聚糖 (EPS) 通过与托尔类受体 (TLRs) 相互作用来调节免疫反应. 这些细菌EPS对树突细胞 (DCs) 中的细胞因子产生表现出结构依赖的影响.
科学领域:
- 微生物学 微生物学
- 免疫学 免疫学 免疫学
- 胃肠病学 胃肠病学
背景情况:
- 益生菌细菌,特别是双倍细菌,产生具有已知的胃肠道益处的外聚糖 (EPS).
- 对于它们在调节宿主免疫反应中的潜在作用,EPS的认可越来越大.
研究的目的:
- 研究来自Bifidobacterium longum亚种的外聚糖 (EPS) 的结构依赖相互作用. 婴儿和青少年的Bifidobacterium具有托尔类受体 (TLRs).
- 确定这些细菌EPS对树突细胞 (DC) 中的细胞因子产生的影响.
主要方法:
- 从B.infantis和B.adolescentis中对EPS的隔离和结构分析.
- 评估EPS与TLR2,TLR4和相关的共受体 (TLR1,TLR6) 的相互作用.
- 在用EPS受条件介质刺激的树突细胞中评估细胞因子 (MCP-1/CCL2,TNFα) 的产生.
主要成果:
- 来自B. infantis和B. adolescentis的EPS表现出不同的结构.
- 这两种EPS类型都以结构依赖的方式抑制了TLR2和TLR4信号.
- 来自B. infantis的EPS显示出较强的TLR2/1抑制,而B. adolescentis的EPS对TLR2/6和TLR4更有效.
- 用EPS调节的介质对DCs的刺激降低了MCP-1/CCL2和TNFα的产生.
结论:
- 来自B. infantis和B. adolescentis的细菌外聚糖具有结构依赖的免疫调节特性.
- 这些EPS影响着树突细胞中的TLR信号传递和细胞因子产生.
- EPS可能作为关键效应分子起作用,调解与食用双菌相关的健康益处.
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