表面蛋白应激对基因表达和Spodoptera litura中的病理变化的影响
Feiyan Zhang1,2,3, Qiuyue Liu1,2,3, Yana Wang1,3
1Institute of Biology, Hebei Academy of Sciences, Shijiazhuang, 050081, People's Republic of China.
Scientific reports
|December 5, 2024
概括
来自Bacillus Vélezensis的表面素通过增加活性氧物种和破坏排毒来损害Spodoptera litura,提供一种潜在的生物杀虫剂替代品. 这项研究揭示了它对昆虫代谢物和基因的影响.
科学领域:
- 农业昆虫学 农业昆虫学
- 生物化学 生物化学
- 分子生物学分子生物学
背景情况:
- 虫 (S. litura) 是一种主要的虫害虫,导致作物损失.
- 传统的化学农药导致环境问题和害虫耐药性.
- 对于环保的生物农药有着至关重要的需求.
研究的目的:
- 为了阐明Bacillus Vélezensis (B. vélezensis) 表面素对S. litura. 的作用机制.
- 研究表面素对S. litura的基因表达和代谢物概况的影响.
- 评估表面素作为一种新型生物杀虫剂的潜力.
主要方法:
- 在S. litura暴露于表面素.
- 转录组和代谢组分析以确定受影响的基因和代谢产物.
- 对S. litura组织的组织病理学检查.
主要成果:
- 表面素显著改变了关键代谢物的表达,包括降低原氨基素 (PPO) 和葡萄糖 (GDL) 的调节,并增加谷氨酸的调节.
- 排毒基因 (谷氨S转移酶 (GST) 和乙甲脱酶 (ALDH)) 和亡抑制基因 (缺席同源1 (SIAH1) 中的七个) 的表达显著下降.
- 在昆虫的肠道,马尔皮奇管和神经细胞中观察到病理变化,表明细胞受损.
结论:
- 表面素诱导氧化应激 (活性氧物种 - ROS) 和S. litura.中的细胞损伤.
- 表面素可能会引起神经毒性和心脏毒性,同时抑制昆虫排毒通路.
- 这些发现支持表面素作为一种有希望的候选人,用于开发新的生物杀虫剂来对抗虫虫害.
相关概念视频
01:17Gene Regulation During Sporulation
Sporulation is a complex developmental process that allows certain Gram-positive bacteria, such as Bacillus subtilis and Clostridium species, to survive extreme environmental conditions. This process is tightly regulated by a series of signaling cascades and transcriptional controls, ensuring the formation of a highly resistant endospore.Sporulation is triggered by unfavorable conditions, such as nutrient depletion, and is governed by a phosphorelay system. One of the sensor kinases, such as...
01:23Stringent Response in E. coli
Bacterial growth is closely tied to nutrient availability, with cells proliferating exponentially under favorable conditions and entering a stationary phase when resources become scarce. This transition is mediated by a regulatory mechanism known as the stringent response, which allows bacteria to adapt to nutrient deprivation by modulating gene expression and metabolic activity.During nutrient scarcity, intracellular amino acid levels decline. It results in the accumulation of uncharged tRNAs...