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储存对使用猪鼻腔粘液的体外透和粘合设置实验的影响
Jaiza Samara Macena de Araújo1, Gabriela Gama Xavier Augusto1, Aylla Mesquita Pestana1
1Department of Biosciences, Faculdade de Odontologia de Piracicaba, Universidade Estadual de Campinas, 901 Limeira Avenue, Bairro Areião, Piracicaba, Sao Paulo, 13414-903, Brazil.
AAPS PharmSciTech
|December 5, 2024
概括
储存猪鼻腔粘膜用于体外研究,损害了其完整性,增加了药物透性,可能导致不准确的配方开发. 对于药物输送研究中可靠的实验结果,建议使用新鲜的鼻腔粘膜.
科学领域:
- 药理学和制药科学 药理学和制药科学
- 生物材料科学 生物材料科学
- 药物输送系统 药物输送系统
背景情况:
- 鼻内药物输送是局部和全身管理的一个有前途的途径.
- 猪鼻腔粘膜通常在体外用于透和粘膜粘合研究.
- 储存对粘膜完整性的影响及其对实验结果的后续影响尚不清楚.
研究的目的:
- 为准备猪鼻腔粘膜在体外研究中的标准化.
- 评估储存条件对猪鼻腔粘膜的完整性和透性的影响.
- 使用纹理分析仪设备优化体外粘粘合实验.
主要方法:
- 猪鼻腔粘膜在各种条件下 (冷藏,冷) 储存,并通过显微镜进行评估.
- 在体外药物透研究中,在弗朗茨型扩散系统中使用利多卡因化物 (LDC) 进行了透研究.
- 在试验室内,使用带有中央复合设计的纹理分析仪对探针速度,接触时间和应用力等变量进行了优化.
主要成果:
- 新鲜组织保持了组织学和超结构完整性.
- 储存的组织显示不组织,减少厚度,并丧失上皮质完整性.
- 在储存的组织中,利多卡因化 (LDC) 的透性显著增加,而粘膜粘合实验需要0.48N的最低接触力以获得最佳结果.
结论:
- 制备技术为透性研究保持粘膜完整性.
- 猪鼻腔粘膜的储存高估了药物透率,可能会扭曲配方开发数据.
- 新鲜的鼻腔粘膜对于准确的体外透性评估至关重要,而特定的参数对于可靠的粘膜粘合测试至关重要.
相关概念视频
01:16Methods for Studying Drug Absorption: In vitro
In vitro experiments are crucial for understanding the transport and absorption of drugs through biological materials. These studies employ varied methods such as the diffusion cell method, the everted sac technique, and the everted ring technique.
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01:09Methods for Studying Drug Absorption: In situ
In situ experiments, such as the Doluisio method and Single-Pass Perfusion technique, provide critical insights into drug uptake by simulating in vivo conditions for drug absorption.
The Doluisio method involves perfusing a prepared segment of a rat's small intestine with a solution of radiolabeled drug and a non-absorbable marker. This helps to differentiate between absorbed and non-absorbed drug concentrations. The intestinal segment is connected at both ends using tubing and syringes,...
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