在硬小麦DR88中分子鉴定Pm4等位基因,使硬小麦DR88具有粉状菌耐药性
Guohao Han1, Lixian Xing1, Tiantian Gu1
1Center for Agricultural Resources Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Shijiazhuang, 050022, China.
BMC plant biology
|December 5, 2024
概括
在 durum小麦中发现了一种新的粉抗性基因PmDR88,并将其映射到染色体2AL上的Pm4位点. 这种基因具有广泛的耐药性,可以用于小麦育种计划中的标记器辅助选择.
科学领域:
- 植物病理学 植物病理学
- 小麦遗传学 小麦遗传学
- 分子育种是指分子育种.
背景情况:
- 粉状菌 (由Blumeria graminis f. sp. 引起) 是一种疾病. 麦 (Triticum) 是对小麦生产的重大全球威胁.
- 硬小麦是一种宝贵的遗传资源,可以增强常见小麦的耐药性.
研究的目的:
- 确定和描述硬麦在硬麦加入DR88.88.中广泛的粉状菌耐药性的遗传基础.
- 开发分子工具,使这种抗性侵入精英小麦品种.
主要方法:
- 大量分离RNA-Seq (BSR-Seq) 用于基因识别.
- 使用分子标记物对F2:3家族进行高通量基因型鉴定.
- 定量实时PCR (qRT-PCR) 用于基因表达分析.
- 序列对齐用于比较分析.
主要成果:
- 一个主要的粉状菌抗性基因,PmDR88,被确定并映射到染色体臂2AL.AL上的Pm4位点.
- PmDR88与Pm4d具有相同的氨基酸序列,但表现出不同的表达模式.
- 两个DNA标记物 (JS717×JS718和WGRE77410) 被验证为标记物辅助选择 (MAS).
结论:
- PmDR88代表了一种有价值的Pm4等位基因,用于增强小麦粉的耐药性.
- 已识别的标记物有助于有效的MAS用于PmDR88的内进.
- 这项研究支持培育努力,以提高小麦对毁灭性疾病的抵抗力.
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