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猪肌膜中的免疫调节动态:全球转录组分析,包括PPARγ配体的影响

Aleksandra Kurzynska¹, Karol Mierzejewski², Monika Golubska²

¹Department of Animal Anatomy and Physiology, University of Warmia and Mazury in Olsztyn, Olsztyn, Poland. aleksandra.kurzynska@uwm.edu.pl.

|December 5, 2024

概括

这项研究揭示了氧酶增殖器激活受体 (PPARγ) 在怀孕期间和雌性周期期间如何影响猪肌体中的基因表达,突出了免疫反应和激素合成中的作用.

科学领域:

生殖生物学生殖生物学
基因组学就是基因组学.
分子内分泌学分子内分泌学

背景情况:

子宫在怀孕和雌性周期期间起着至关重要的作用.
过氧体增殖器激活受体 (PPARs) 是肌体过程的潜在调节者.
在关键生殖阶段缺少猪骨髓体的全球转录组概况.

研究的目的:

为了确定猪肌体在周植入期和晚期黄体阶段的全球转录组概况.
为了研究PPARγ配体对肌胎转录组的影响.
在响应PPARγ调制时识别差异表达基因 (DEGs).

主要方法:

在怀孕 (10-11日和14-15日) 和雌性周期期间采集的猪肌膜样本.
用PPARγ激动剂 (皮奥格利塔) 和对抗剂 (T0070907) 进行肌肉细胞扩散体体内化.
使用Illumina NovaSeq 6000进行转录组分析的RNA测序 (RNA-Seq).

主要成果:

在怀孕和雌性周期阶段之间,在猪肌体中确定了1082个DEG.
在怀孕和雌性周期期间检测到对PPARγ激动剂和对抗剂的显著基因表达变化.
具体来说,分别有6个和80个DEG与怀孕期间的PPARγ激动剂和对抗剂有关.

结论:

不同表达的基因参与免疫反应,前列腺素合成,细胞分化和通信.
PPARγ活性在调节与免疫反应和猪骨髓中的激素合成相关的基因方面发挥着重要作用.
这项研究为管理生殖过程中肌肉功能的分子机制提供了新的见解.

关键词:

细胞因子细胞因子免疫反应的免疫反应.皮奥格利塔是如何使用的 T0070907 时间时间时间转录因子转录因子

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