报告的患病率和Candida africana的诊断方法的比较:通过元分析进行系统性审查
Bwambale Jonani1,2, Emmanuel Charles Kasule3, Herman Roman Bwire3
1Department of Immunology and Molecular Biology, School of Biomedical Sciences, College of Health Sciences, Makerere University, P.O. Box 7072, Kampala, Uganda. bwambale.jonani@mak.ac.ug.
Diagnostic and prognostic research
|December 5, 2024
概括
这次审查发现Candida africana在0.9%的人群中存在,主要来自阴道样本. 像HWP1 PCR这样的分子方法是识别这种酵母的最佳方法,因为其他系统通常无法将其与Candida albicans区分开来.
科学领域:
- 医学真菌学 医学真菌学
- 传染性疾病 传染性疾病
- 微生物学 微生物学
背景情况:
- 非洲是一种机会主义的酵母.
- 它与阴道和口腔候群病有关.
- 准确的鉴定对于有效的治疗至关重要.
研究的目的:
- 为了评估非洲菌的流行情况.
- 评估用于识别非洲菌的诊断方法.
- 为了比较不同诊断技术的准确性.
主要方法:
- 53项研究的系统审查和元分析.
- 从20571名参与者中进行了聚合流行率计算.
- 对诊断方法的分析,包括HWP1 PCR,Vitek-2和MALDI-TOF.
主要成果:
- 非洲菌的综合流行率为0.9% (95% CI:0.7-1.3%).
- 在北美观察到的患病率最高 (4.6%).
- 大多数隔离 (84.52%) 来自阴道样本.
- HWP1 PCR 是最常用的诊断方法 (70%).
- 维特克-2未能将C. africana与Candida albicans区分开来.
- 与HWP1 PCR相比,MALDI-TOF显示出了错误的识别.
结论:
- 非洲菌的流行率低但显著,特别是在阴道感染中.
- 像HWP1 PCR这样的分子方法对于准确的识别是优越的.
- 目前的自动化系统 (Vitek-2,MALDI-TOF) 在区分C. africana.方面存在局限性.
- 需要开发可访问的诊断替代方案,特别是在资源有限的环境中.
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