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完整的基因组序列的Magnaporthe oryzae 聚密病毒 1 的基因组序列

Hong Zheng¹, Cong Li¹, Yuxin Wu¹

¹College of Agriculture, Yangtze University, Jingzhou, 434025, Hubei, China.

|December 6, 2024

概括

在米爆真菌中发现了一种新的真菌病毒,Magnaporthe oryzae polymycovirus 1 (MoPmV1). 这种双链RNA病毒扩大了众所周知的多种多样性.

科学领域:

* 菌群学 * 菌群学
* 病毒学病毒学
* 分子生物学 * 分子生物学

背景情况:

* 菌,如大米爆发真菌 *Magnaporthe oryzae*,可以庇护各种病毒.
* 菌类病毒,特别是双链RNA (dsRNA) 病毒,在真菌生物学和进化中发挥作用.
*Polymycoviridae家族包括具有细分基因组的dsRNA病毒,但它们在植物病原菌中的多样性仍未得到充分探索.

研究的目的:

* 为了识别和描述Magnaporthe oryzae*中的新型真菌病毒.
* 确定一个新发现的dsRNA病毒的基因组特征和系遗传关系.
*将病毒分类到现有的病毒分类系统中,特别是Polymycoviridae家族.

主要方法:

*从TM02菌株Magnaporthe oryzae*中分离和净化dsRNA.
*下一代dsrna片段的测序和组装,以确定基因组组织.
*生物信息分析,包括蛋白质同质性搜索的BLASTp和保存域的基因分析 (例如,依赖RNA的RNA聚合酶).

主要成果:

*鉴定了一种新的dsRNA病毒,被指定为Magnaporthe oryzae polymycovirus 1 (MoPmV1),具有四个dsRNA段 (13242401 bp).
*MoPmV1 dsRNA片段编码了假定RNA依赖的RNA聚合酶 (RdRp),蛋白酶,甲基转移酶和富含氨酸和氨酸的蛋白质 (PASr),它们的序列与已知的病毒蛋白质相同.
*基于RdRp域的家族遗传学分析将MoPmV1置于*Polymycovirus*属*Polymycoviridae*家族内.

结论:

*MoPmV1代表了属 *Polymycovirus* *Polymycoviridae* *家族*中的一个新物种.
* 这是第一次报告在米爆真菌*Magnaporthe oryzae*中发现的Polymycoviridae家族的一种真菌病毒.
*发现MoPmV1有助于了解植物病原菌的病毒圈和多种病毒的进化多样性.

关键词:

马格纳波特花 (Magnaporthe oryzae) 是一个很大的植物.这种病毒是Polymycoviridae.这就是Mycovirus.大米炸弹爆炸

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