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在固体瘤中构建和描述一种新型分泌的MsC-CAR-T细胞

Yuan Mao¹, Yufeng Chen², Xiaohui Yang³

¹Department of Geriatric Oncology, The Fourth Affiliated Hospital of Nanjing Medical University, Nanjing, China; National Health Commission Key Laboratory of Antibody Techniques, Nanjing Medical University, Nanjing, China; Department of Pathology, Nanjing Medical University, Nanjing, China; Jiangsu Province Engineering Research Center of Antibody Drug, Nanjing, China.

|December 6, 2024

概括

这项研究引入了针对MAGE-A1并分泌CD47阻断抗体的新型工程T细胞 (MsC1-CART). 这些细胞通过结合免疫疗法和免疫检查点阻塞,显示出治疗MAGE-A1阳性固体瘤的巨大潜力.

科学领域:

免疫学免疫学免疫学
在瘤学瘤学.
生物技术是生物技术.

背景情况:

CD47-SIRPα信号传递在固体瘤中起到至关重要的免疫检查点的作用.
CD47阻断是固体瘤的一种有前途的治疗策略.
对于固体瘤的CAR-T细胞和抗体碎片分泌的联合使用尚未得到充分研究.

研究的目的:

在实体瘤中使用生物信息数据库调查CD47特征.
设计,优化和构建一个针对MAGE-A1和分泌CD47-scFv的新型MsC-CAR.
在体外和体内评估工程T细胞 (MsC1-CART) 的瘤抑制功效.

主要方法:

在固体瘤中搜索CD47特征的生物信息数据库.
设计和制造一种新的MAGE-A1,针对具有自我分泌CD47-scFv (MsC-CAR) 的CAR.
工程T细胞 (MsC-CART) 的转染,验证和表征及其抗瘤活性的评估.

主要成果:

成功构建MsC-CARs,MsC1-CARs显示有效的MAGE-A1识别和CD47-scFv分泌.
MsC1-CART细胞在各种癌症细胞系和LUAD异种移植模型中表现出显著的瘤抑制有效性.
设计的 MsC1-CART 细胞有效地将采用细胞免疫疗法与免疫检查点抑制剂疗法结合起来.

结论:

MsC1-CART细胞代表了MAGE-A1阳性固体瘤的新疗法.
该策略将CAR-T细胞疗法与免疫检查点抑制相结合,以增强抗瘤活性.
MsC1-CART细胞为治疗MAGE-A1阳性固体瘤提供了一个有希望的新方向.

关键词:

在CART-T中,我们可以使用CAR-T.CD47 CD47 CD47 CD47 CD47 CD47 CD47 CD47 CD47 CD47 CD47 CD47 简介:MAGE-A1 一个机密的秘密的秘密的

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