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Updated: Jun 5, 2025

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies
10:01

Combining Chemical Cross-linking and Mass Spectrometry of Intact Protein Complexes to Study the Architecture of Multi-subunit Protein Assemblies

Published on: November 28, 2017

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整合分子动力学模拟 解交联数据 揭示线粒体蛋白质分布

Fabian Schuhmann1, Kerem Can Akkaya2,3, Dmytro Puchkov3

  • 1Niels Bohr Institute, University of Copenhagen, Blegdamsvej 17, 2100, Copenhagen, Denmark.

Angewandte Chemie (International ed. in English)
|December 7, 2024
PubMed
概括

这项研究将交叉连接质谱法 (XL-MS) 与模拟相结合,绘制线粒体蛋白相互作用图. 该方法澄清了复杂的数据,识别了蛋白质集群,并精确地定位了线粒体内的蛋白质.

关键词:
3D体积成像 3D体积成像交叉链接质谱仪的质谱仪.线粒体蛋白质的分布分子动力学分子动力学上面是粗粒度的

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Translocation of Proteins into the Mitochondria01:19

Translocation of Proteins into the Mitochondria

Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
Sorting of outer membrane proteins:
Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
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