拉普-马尔迪 (LAP-MALDI MS) 分析牙中的阿梅洛金因,以估计生物性别
Lily R Adair1, Mary E Lewis2, Matthew J Collins3
1Department of Chemistry, University of Reading, Whiteknights, Reading RG6 6DX, United Kingdom.
Journal of pharmaceutical and biomedical analysis
|December 7, 2024
概括
这项研究引入了一种使用牙面膜蛋白进行生物性别估计的快速方法. 新的工作流大大缩短了分析时间到不到10分钟,提高了法医和考古应用的可访问性.
科学领域:
- 法医科学 法医科学 法医科学
- 生物化学 生物化学
- 蛋白质组学是指蛋白质组学.
背景情况:
- 在法医和考古学背景下,生物性别估计至关重要.
- 目前使用LC-ESIMS/MS进行阿梅洛金蛋白分析的方法是耗时的.
- 在性染色体 (X和Y) 上存在阿梅洛基因基因,可以确定性别.
研究的目的:
- 开发一个快速的,无LC的工作流程,用于生物性别估计.
- 通过使用LAP-MALDIMS/MS.来分析阿梅洛金来确定性别.
- 以蛋白质学见解为目的,对其他杏仁素和杏仁母细胞蛋白进行表征.
主要方法:
- 从牙面膜中微酸提取阿梅洛根因酸.
- 液体大气压矩阵辅助激光吸附/电离 (LAP-MALDI) 的MS和MS/MS分析.
- 没有液态色谱 (LC) 分离步骤的分析.
主要成果:
- 一个工作流程,使生物性别估计在≤10分钟内,MS/MS获取为1分钟.
- 对Y染色体特定的SMoxIRPPY的鉴定.
- 对27种杏仁素和杏仁芽蛋白的表征,揭示了翻译后的修改.
结论:
- 开发的LAP-MALDI工作流提供了一个快速有效的替代方案来估计生物性别.
- 这种方法具有最小的危险性,适用于非专业化实验室.
- 工作流提供了进一步对牙面膜蛋白质进行蛋白质组分析的机会.
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