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便携式食品传染病原体检测通过比度光纳米探针用于基于DNA功能化的金属有机框架的腺三酸盐量化

Changling Li¹, Yingjie Huang², Shenghua Li¹

¹Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province, College of Biological and Food Engineering, Huaihua University, Huaihua 418008, PR China.

International journal of biological macromolecules

|December 7, 2024

概括

一个新的便携式纳米探测器检测腺三酸盐 (ATP),一个活性的细菌标记,使用比度光. 这种方法可以快速,灵敏地在现场检测牛奶中的食物传播病原体.

科学领域:

食品科学食品科学食品科学
分析化学分析化学
生物技术是生物技术.

背景情况:

食物传播疾病是一个日益关注的问题,需要先进的检测方法.
目前的病原体检测方法可能是缓慢的,复杂的,需要实验室设置.
氨酸三酸盐 (ATP) 是可靠的生物标志物,用于可活的细菌细胞.

研究的目的:

开发一种便携式,灵敏和快速的方法来量化ATP.
为精确的ATP检测创建一个比度光纳米探针.
建立一个对食物传播病原体的现场检测套件.

主要方法:

使用基金属有机框架封装Ru(bpy) 32+并与dsDNA功能化构建一个比度光纳米探针.
使用SYBR绿色I (SGI) 插入dDNA进行双发射光.
开发一个便携式水凝套件,将纳米探针集成到现场检测和基于智能手机的色彩识别中.

主要成果:

纳米探测器实现了以0.63μM的检测极限 (LOD) 的比度ATP检测.
综合水凝套件在牛奶中检测病原体具有很高的灵敏度,LOD为10 CFU/mL.
智能手机应用程序实现了快速准确的色度分析.

结论:

开发的比度光纳米探针和水凝套件为食品安全中的实时细菌检测提供了一个有前途的解决方案.
该套件的便携性和敏感性使其能够在现场进行监测,并对潜在的食品传播污染作出快速反应.
这项技术在改善食品安全监督和公共卫生保护方面具有重大潜力.

关键词:

亚丁酸三酸盐是一种氨酸三酸盐.光纳米探针是一种光纳米探针.通过食物传播的致病原体凝套件凝套件金属有机框架的框架.

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