奇托/瓦尼林/多甲基素支架具有可调节的硬度,用于肌肉细胞的增殖
Rafael Leonardo Cruz Gomes da Silva1, Dragica Bezjak2, Tomas P Corrales3
1Fundamental Chemistry Department, Institute of Chemistry, University of São Paulo, Av. Prof. Lineu Prestes 748, 05508-000 São Paulo, Brazil.
International journal of biological macromolecules
|December 7, 2024
概括
这项研究开发了可调节的奇托桑支架,使用聚甲基西洛 (PDMS) 为C2C12核细胞. 脚手架表现出调制的机械性能和良好的细胞粘附性,素PDMS表现出比基于素的更好的生存能力.
科学领域:
- 生物材料科学 生物材料科学
- 组织工程是组织工程.
- 聚合物化学 聚合物化学
背景情况:
- 脚手架的机械特性极大地影响了细胞的行为.
- 基托和素交联基托是用于组织再生的有希望的生物材料.
- 定制脚手架机制对于优化细胞反应至关重要.
研究的目的:
- 开发一种方法来生产具有可调整机械性能的基托和素交联基托薄膜.
- 为了研究聚甲基西洛 (PDMS) 弹性体结合对脚手架机械学的影响.
- 评估这些支架适用于C2C12神经细胞应用的适用性.
主要方法:
- 使用聚酸盐20制备水性PDMS弹性散物.
- 将PDMS分散物纳入基托或瓦尼林交联基托聚合物分散物中.
- 通过造和使用原子力显微镜 (AFM) 在力光谱模式下表征膜的制造.
- 评估机械性能 (弹性模量),胀程度,凝含量,细胞活力和细胞粘附.
主要成果:
- PDMS的整合成功调节了基托支架 (60200 kPa) 和素基支架 (200600 kPa) 的弹性模量 (E).
- 更软的支架表现出更高的胀和更低的凝含量.
- 桑-PDMS支架显示出良好的细胞活力和C2C12髓细胞粘附在一系列弹性模块 (114568 kPa).
- 基于香的支架显示了边界细胞毒性 (~70%的活力).
结论:
- 建立了一种新的方法,可以从基托和素交联基托中创建可调节的机械性质支架.
- 在组织工程应用中,PDMS弹性体的结合提供了一个可行的策略来控制支架机制.
- 酸盐-PDMS支架显示出C2C12髓母细胞培养的潜力,这是由于有利的机械特性和生物相容性.
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