相关实验视频

Updated: Jun 5, 2025

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Characterization of a Pathogenic Escherichia coli Strain Derived from Oreochromis spp. Farms Using Whole-Genome Sequencing

Published on: December 23, 2022

禽病原体大肠杆菌O1:K1和O2:K1的演变和动物感染风险

Eun-Jin Ha¹, Seung-Min Hong¹, Kang-Seuk Choi¹

¹Laboratory of Avian Diseases, College of Veterinary Medicine and BK21 PLUS for Veterinary Science, Seoul National University, Seoul, 088026, Republic of Korea; Research Institute for Veterinary Science, College of Veterinary Medicine, Seoul, 08826, Republic of Korea.

Microbes and infection

|December 7, 2024

概括

禽类致病性大肠杆菌 (APEC) 和人类肠外致病性大肠杆菌 (huExPEC) 具有共同的进化联系. 这项研究表明huExPEC传染给家禽,进化为毒性APEC菌株,引发动物感染的担忧.

科学领域:

微生物学微生物学
进化生物学进化生物学
基因组学就是基因组学.

背景情况:

鸟类致病性大肠杆菌 (APEC) 和人类肠外致病性大肠杆菌 (huExPEC) 的O1和O2血清组呈现出密切的关系.
进一步阐明它们的进化联系对于理解病原体传播和公共卫生风险至关重要.

研究的目的:

研究O1和O2 APEC和huExPEC之间的进化关系.
使用rpoB序列类型 (RST) 分类APEC菌株并与huExPEC进行比较.

主要方法:

分子prophage类型的编号
病毒性和抗生素耐药性基因分析.
进行比较的基因组学.
rpoB 序列类型化 (RST) 技术

主要成果:

大多数O1:K1和O2:K1APEC菌株属于RST46-1和RST47-9.
韩国O1 APEC独有的RST47-9,可能起源于RST46-1 APEC.
对比基因组学表明O1和O2抗原转移到人类RST46-1大肠杆菌,在那里K1囊出现.
韩国APEC和huExPEC共享CRISPR间隔器,但在抗生素图和prophage含量上有所不同.
有证据表明RST46-1 huExPEC传播到家禽中并进化到家禽中.

结论:

RST46-1 huExPEC可能传播给家禽,进化为毒性APEC菌株.
在家禽中,huExPEC的逆 zoonosis 传播潜力令人担忧.
虽然动物传染病风险尚不清楚,但这些APEC菌株的毒性突出显示了潜在的公共卫生威胁.

关键词:

进化进化进化进化进化进化进化在ExPEC中使用ExPEC.在K1囊中.分子prophage类型的编号 O1/O2 亚太经合组织这是一种逆转的动物感染.rpoB 序列的输入方式

更多相关视频

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Loop-mediated Isothermal Amplification LAMP Assays for the Species-specific Detection of Eimeria that Infect Chickens

Published on: February 20, 2015

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Nucleofection and In Vivo Propagation of Chicken Eimeria Parasites

Published on: February 14, 2020

相关概念视频

Viral Recombination

Viral Recombination

Cells are sometimes infected by more than one virus at once. When two viruses disassemble to expose their genomes for replication in the same cell, similar regions of their genomes can pair together and exchange sequences in a process called recombination. Alternatively, viruses with segmented genomes can swap segments in a process called reassortment.

Viral Mutations

Viral Mutations

A mutation is a change in the sequence of bases of DNA or RNA in a genome. Some mutations occur during replication of the genome due to errors made by the polymerase enzymes that replicate DNA or RNA. Unlike DNA polymerase, RNA polymerase is prone to errors because it is not capable of “proofreading” its work. Viruses with RNA-based genomes, like HIV, therefore accrue mutations faster than viruses with DNA-based genomes. Because mutation and recombination provide the raw material...

Infection

Infection

When a pathogen enters the body and reproduces, it can cause an infection, damage body cells, and cause illness symptoms that eventually lead to disease. Therefore, its prevention requires breaking the chain of infection.
The chain begins with pathogens: bacteria, viruses, fungi, prions, or parasites such as protozoa helminths. These can be present on the skin as transient or resident flora, or they can be acquired from the environment. Identifying and treating the type of infection and...