基因组H4K8hib的修饰促进了基因表达,并调节了大米免疫力
Qiutao Xu1, Xuan Ma2, Xuelu Wei1
1State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Agriculture, Guangxi University, Nanning 530004, China.
Molecular plant
|December 8, 2024
概括
米表观遗传学研究显示,H4K8hib是基因表达和免疫力的关键标记物. 酶HDA705调节了这种标记,增强了植物对病原体的防御,当病原体发生突变时.
科学领域:
- 植物分子生物学 植物分子生物学
- 表观遗传学 在表观遗传学中,表观遗传学是指表观遗传学.
- 植物免疫力 植物免疫力
背景情况:
- 表观遗传修饰在调节植物的基因表达和应激反应方面发挥着至关重要的作用.
- 质子修饰是表观遗传调节的核心,但某些标记的特定作用,如H4K8hib,在米中仍未得到充分探索.
- 了解表观遗传机制对于提高作物弹性和抗病能力至关重要.
研究的目的:
- 调查H4K8hib作为大米中活性表观遗传标记的功能.
- 确定负责调节H4K8hib水平的关键酶.
- 确定H4K8hib对大米基因表达,免疫反应和病原体耐药性的影响.
主要方法:
- 染色体免疫沉 (ChIP) 测定检测H4K8抑制水平.
- 使用定量实时PCR (qRT-PCR) 进行基因表达分析.
- 为HDA705基因生成和分析米突变物.
- 病原体挑战测试用于评估疾病耐药性.
主要成果:
- H4K8hib被确定为大米中的活性表观遗传标记,与基因表达有积极的相关性.
- 鉴定出HDA705是调节H4K8hib.的主要酶.
- HDA705的突变导致H4K8hib水平增加 (超H4K8hib).
- 超H4K8hib激活了与防御相关的基因的表达,并显著增强了大米对病原体的抵抗力.
结论:
- H4K8hib作为大米中的关键活性表观遗传调节剂,影响基因表达和天生的免疫力.
- HDA705是控制H4K8hib的关键表观遗传调节剂,从而调节植物防御通路.
- 针对HDA705-H4K8hib轴提供了增强作物抗病能力的潜在策略.
相关概念视频
02:32Histone Modification
13.0K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
13.0K
01:32Chromatin Modification in iPS Cells
1.6K
Chromatin modification alters gene expression; therefore, scientists can add histone-modifying enzymes, histone variants, and chromatin remodeling complexes to somatic cells to aid reprogramming into pluripotent stem (iPS) cells.
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
Compact chromatin makes reprogramming difficult. Enzymes, such as histone demethylases and acetyltransferases, are often added during reprogramming to loosen the chromatin, making the DNA more accessible to transcription factors. Molecules that inhibit histone...
1.6K
01:23Regulation of Expression at Multiple Steps
869
The gene expression in cells is regulated at different stages: (i) transcription, (ii) RNA processing, (iii) RNA localization, and (iv) translation. Transcriptional regulation is mediated by regulatory proteins such as transcription factors, activators, or repressors—these control gene expression by initiating or inhibiting the transcription of genes. Once a precursor or pre-mRNA is produced, it undergoes post-transcriptional modification, including 5' capping, splicing, and the...
869
02:25Spreading of Chromatin Modifications
8.2K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
Writers
The writer...
8.2K
02:41Chromatin Structure Regulates pre-mRNA Processing
6.9K
In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
The chromatin structure, especially...
6.9K
01:37Epigenetic Regulation
3.0K
Epigenetic changes alter the physical structure of the DNA without changing the genetic sequence and often regulate whether genes are turned on or off. This regulation ensures that each cell produces only proteins necessary for its function. For example, proteins that promote bone growth are not produced in muscle cells. Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
X-chromosome...
X-chromosome...
3.0K