对Paspalum notatum的转录基因导向育种:产生具有增强omega-3含量的apomictic杂交物
Lara Marino1, Silvia Altabe2, Carolina Marta Colono1
1Instituto de Investigaciones en Ciencias Agrarias de Rosario (IICAR-CONICET-UNR), Parque Villarino S/N, Z2125ZAA Zavalla, Rosario, Santa Fe, Argentina.
概括
在Paspalum notatum的阿波米克斯繁殖创造了具有克隆繁殖和更高阿尔法-烯酸含量的混合物. 这提高了牲畜产品的营养,并可能减少放牧动物的甲排放.
科学领域:
- 植物育种和遗传学
- 营养生物化学 营养生物化学
- 动物科学动物科学
背景情况:
- 牛奶脂肪中低的omega-6:omega-3脂肪酸比率改善了人类的健康.
- 料脂质成分影响反动物的牛奶和甲排放.
- 开发具有高阿尔法-烯酸的apomictic Paspalum notatum是可取的.
研究的目的:
- 为了开发具有高阿尔法-烯酸 (18:3) 含量的Paspalum notatum基因型.
- 在这些基因型中通过种子 (apomixis) 实现克隆繁殖.
- 评估改善畜牧产品质量和减少甲排放的潜力.
主要方法:
- 转录组组合和Paspalum notatum的分析.
- 使用qPCR对脂质代谢基因 (SDP1,PXA1) 的鉴定和表达分析.
- 使用FAME-GC-MS进行脂肪酸分析.
- 性基因型和类基因型之间的杂交.
主要成果:
- 在多倍体性基因型 (Q4188) 中观察到SDP1和PXA1的减少表达.
- 基因型Q4188表现出增加的阿尔法-烯酸 (18:3) 含量.
- 两个阿波米克特F1杂交物 (JS9,JS71) 产生了降低SDP1/PXA1,高于18:3,并产生了克隆繁殖.
结论:
- 阿波米克斯繁殖成功地产生了Paspalum notatum,具有增强的营养特征.
- 这些杂交品种具有提高牛奶和肉质量的潜力.
- 开发的基因型可能有助于减少放牧牲畜中的甲排放.
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