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迪布甲酸通过线粒体介导的细胞功能障碍对热囊细胞产生有毒作用

Hyewon Jang¹, Gwonhwa Song², Whasun Lim¹

¹Department of Biological Sciences, College of Science, Sungkyunkwan University, Suwon 16419, Republic of Korea.

Toxicology and applied pharmacology

|December 8, 2024

概括

丁丁甲酸及其代谢物单丁甲酸会损害 trofhoblast 的功能. 暴露会削弱胎盘屏障,可能会影响怀孕的结果.

科学领域:

生殖毒理学生殖毒理学
环境健康环境健康
细胞生物学细胞生物学

背景情况:

二甲基甲酸盐 (DBP) 是消费品中常见的增塑剂.
已知DBP的生殖毒性,但其在怀孕期间的影响尚不清楚.
热囊细胞损伤与孕前有关,这是一个妊娠并发症.

研究的目的:

为了研究迪布甲对人类热细胞的毒性作用.
阐明DBP通过哪些机制影响热囊细胞功能.
评估DBP代谢产物单丁甲酸 (MBP) 对 trofhoblast 细胞的影响.

主要方法:

使用了暴露于DBP.的人类热细胞系 (HTR-8/SVneo,JEG-3).
评估了 trofhoblast 的活力,入侵和线粒体功能.
分析了PI3K/MAPK信号通路和与自相关的蛋白质 (ATG5,LC3B,SQSTM1/p62).
评估了MBP对细胞移动性,透和自的影响.

主要成果:

DBP显著降低了 trofhoblast 的活力和入侵.
DBP诱导了线粒体去极化,并调节了关键信号通路.
治疗DBP激活了自和损害了 trofhoblast 功能.
此外,MBP还损害了热囊细胞功能,并削弱了热囊细胞屏障.

结论:

DBP诱导自并损害了热囊细胞的功能.
DBP及其代谢物MBP对热囊细胞屏障的完整性构成风险.
这些发现突出了DBP作为怀孕期间潜在的危险.

关键词:

自自是一种自的过程.积累的积累的.双乙烯甲酸二甲基的使用方法胎盘胎盘胎盘胎盘热细胞热细胞

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