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益生菌和后生菌干扰对临床抗甲素耐药黄金葡萄球菌 (MRSA) 具有抗粘附作用,并阻碍HT-29细胞系中MRSA诱导的肠表皮超透性

Basavaprabhu Haranahalli Nataraj¹, Chette Ramesh², Rashmi Hogarehalli Mallappa¹

¹Molecular Biology Unit, Dairy Microbiology Division, ICAR-National Dairy Research Institute, Karnal, 132001, Haryana, India; Dairy Bacteriology Section, Southern Regional Station, ICAR-National Dairy Research Institute, Adugodi, 560030, Bengaluru, Karnataka, India.

Microbial pathogenesis

|December 8, 2024

概括

与肠道细胞上的活益生菌相比,热杀死益生菌 (后益生菌) 对抗生素耐药细菌 (MRSA) 具有优越的抗粘附作用. 这项研究支持益生菌和后生菌作为抗生素对抗感染的潜在替代品.

科学领域:

微生物学微生物学
细胞生物学细胞生物学
胃肠病学胃肠病学

背景情况:

抗生素耐药性,特别是抗甲素黄金葡萄球菌 (MRSA),构成了全球健康的重大威胁.
迫切需要新的战略来打击由抗生素耐药性病原体引起的感染.
益生菌和后生菌为控制病原体生长和感染提供了有希望的途径.

研究的目的:

评估特定益生菌菌株及其热杀菌制剂 (后生物) 与HT-29细胞系上临床MRSA分离物的抗粘附性质.
为了比较活益生菌与后益生菌在预防MRSA粘附方面的疗效.
评估益生菌干预对肠道屏障完整性的影响.

主要方法:

使用的本地 (Limosilactobacillus fermentum Lf1,Lactiplantibacillus plantarum A5) 和参考 (Lacticaseibacillus rhamnosus GG,Lactobacillus acidophilus NCFM) 益生菌菌株及其热杀死后生物制剂.
在HT-29人类结肠腺癌细胞系上对临床MRSA分离物 (MRSA12/206, 5/255) 进行了抗粘附疗效测试.
使用ATR-FTIR进行后生物特征和FITC-dextran测定以评估肠道屏障功能.

主要成果:

活益生菌和后益生菌都通过保护性,竞争性和位移机制显著 (p <0.05) 减少了MRSA对HT-29细胞的粘附.
后生菌与活益生菌相比,表现出更高的抗粘合效果,这归因于微生物相关分子模式的可访问性.
益生菌联合治疗显著降低了FITC-德克斯透流,表明改善了肠道屏障功能.

结论:

在防止MRSA粘附到肠道细胞方面,热杀死益生菌 (后生菌) 比活益生菌更有效.
益生菌和后生菌显示出作为抗生素替代品的潜力,用于管理MRSA殖民和支持肠道健康.
需要进一步的临床前和体外研究来验证这些干预措施的治疗潜力.

关键词:

抗粘合性抗粘合性抗粘合性肠道屏障保护保护肠道屏障保护热量杀死了后生物的生物.在MRSA中,MRSA可能是MRSA.益生菌益生菌益生菌

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