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皮质otropin释放因子类利尿激素44和Drosophila suzukii中的五个相应的GPCR:结构和功能表征

Hojung Yoon¹, Briana Price², Ryssa Parks²

¹USDA-ARS, Horticultural Crops Disease and Pest Management Research Unit, USA; Department of Horticulture, Oregon State University, 3420 NW Orchard Ave, Corvallis, OR 97330, USA.

Journal of insect physiology

|December 8, 2024

概括

研究人员在Drosophila suzukii中确定了类似于皮质otropin释放因子 (CRF) 的DH44及其受体. DH44调节液体分泌和睡眠-清醒周期,DH44和DH31结合增加死亡率,为害虫管理提供了洞察力.

科学领域:

昆虫学和分子生物学
昆虫生理学和内分泌学.
虫害管理策略虫害管理策略

背景情况:

利尿激素 (DHs) 和它们的G蛋白合受体 (GPCRs) 对于关节动物的水和离子稳态至关重要.
昆虫具有两个主要的DHs:类似于素 (CT) 的DH31和类似于皮质otropin释放因子 (CRF) 的DH44.
果虫Drosophila suzukii依赖其DH系统进行生理调节.

研究的目的:

为了识别和表征DH44及其同类GPCRs在Drosophila suzukii.
阐明DH44的生理作用,包括其参与利尿活性和潜在的睡眠-清醒周期调节.
探索DH对于管理Drosophila suzukii种群的潜力.

主要方法:

在Drosophila suzukii中识别和表征DH44和五种GPCR变异 (DH44-R1和DH44-R2a/b/c/d).
对GPCR结构,对DH44的反应和第二信使通路 (cAMP,Ca2+) 的分析.
基因表达分析以确定组织特异性局部 (大脑,马尔皮吉管) 和功能测试,涉及注射.

主要成果:

确定DH44-R1是最长的GPCR,对DH44显示出强烈的反应;DH44-R2变异较短且高度相似.
DH44-R1主要表达在大脑中,而DH44-R2的变体集中在消化器官,特别是马尔皮格管道.
DH44注射刺激了剂量依赖的液体分泌;同时注射DH31和DH44导致高死亡率.

结论:

在Drosophila suzukii中,DH44在调节利尿活性和潜在的睡眠-清醒周期方面发挥着重要作用.
DH44受体的差异性表达表明不同组织中的特殊功能.
DH系统,特别是DH31和DH44的协同作用,为开发针对Drosophila suzukii的新害虫管理策略提供了潜在的目标.

关键词:

利尿激素利尿激素利尿激素这种植物是Drosophila suzukiiii.G蛋白结合受体的受体是G蛋白结合受体的受体.马尔皮吉亚管状管体神经是一种神经.在CAMP中,我们可以使用cAMP.

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