相关实验视频

Updated: Jul 17, 2026

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Construction of Vapor Chambers Used to Expose Mice to Alcohol During the Equivalent of all Three Trimesters of Human Development

Published on: July 13, 2014

产前酒精中毒动物的神经免疫特征

Inna Yu Shamakina¹, Petr K Anokhin^2,3, Ruslan A Ageldinov⁴

¹Serbsky National Medical Research Center of Psychiatry and Narcology, Ministry of Health of the Russian Federation, Moscow, 119002, Russia. shamakina.i@serbsky.ru.

Biochemistry. Biokhimiia

|December 8, 2024

概括

产前酒精暴露会改变成年大鼠大脑中的神经炎症标志物. 这些对细胞因子和化学因子的影响是性别特异的,对男性的影响比女性更大.

科学领域:

神经科学是一个神经科学.
免疫学免疫学免疫学
发展生物学发展生物学

背景情况:

产前酒精暴露 (PAE) 与中枢神经系统 (CNS) 疾病有关,包括认知和情感问题.
神经炎症在这些PAE相关疾病的病理生理学中发挥着关键作用.

研究的目的:

研究PAE对成年大鼠前额叶皮层中神经免疫因子的性别特异性影响.
在子宫内暴露于酒精后分析关键细胞因子和化学因子的水平.

主要方法:

使用Wistar老鼠在妊娠期间消耗乙醇的PAE的动物模型.
采用多重免疫光学来量化互白蛋白的蛋白质水平 (IL-1α,IL-1β,IL-3,IL-6,IL-9,IL-12),瘤缩因子-α (TNF-α) 和CCL5.5.
应用定量实时PCR测量细胞因子信使RNA (mRNA) 在前额叶皮层的水平.

主要成果:

在男性后代的前额皮层中,PAE导致TNF-α,IL-1β,IL-3,IL-6和IL-9蛋白水平显著下降.
在女性后代中没有观察到这些下降,这表明性别特异性反应.
PAE男性的TNF-αmRNA水平降低了45%,这表明转录调节.

结论:

产前酒精暴露对前额叶皮层内的神经免疫因子调节以性别特定的方式产生差异性影响.
这些发现强调了在胎儿酒精谱系障碍 (FASD) 的转化研究中将性别视为生物变量的重要性.
了解这些特定于性别的神经炎症变化对于制定针对性预防和治疗FASD策略至关重要.

关键词:

它们包括interleukins和interleukins.mRNA的表达方式神经炎症是一种神经炎症.前额叶皮层前额叶皮层.产前酒精中毒产前酒精中毒瘤死亡因子是瘤死亡因子.

更多相关视频

Moderate Prenatal Alcohol Exposure and Quantification of Social Behavior in Adult Rats

Moderate Prenatal Alcohol Exposure and Quantification of Social Behavior in Adult Rats

Published on: December 14, 2014

The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders

The Use of Trace Eyeblink Classical Conditioning to Assess Hippocampal Dysfunction in a Rat Model of Fetal Alcohol Spectrum Disorders

Published on: August 5, 2017

相关概念视频

Rabies

Rabies

Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...

Encephalitis ll: Pathophysiology

Encephalitis ll: Pathophysiology

Encephalitis is inflammation of the brain parenchyma caused by direct viral invasion or immune-mediated mechanisms triggered by infections or tumors. Both processes lead to neuronal injury, disrupted neurotransmission, and diverse neurological symptoms, often with overlapping clinical and pathological features.Autoimmune EncephalitisIn autoimmune encephalitis, antibodies target neuronal antigens on cell surfaces, synapses, or within neurons. A key example is anti-NMDAR encephalitis, which can...