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相关概念视频

Glycocalyx and its Functions01:14

Glycocalyx and its Functions

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The glycocalyx is a carbohydrate-rich, fuzzy-appearing layer on the outer surface of the cell membrane. It is highly hydrophilic, because of this it attracts large amounts of water to the cell's surface. This aids the cell's interaction with the watery environment and also helps it to obtain substances dissolved in the water. It is also important for cell identification, self/non-self determination, and embryonic development and is used in cell-to-cell attachments to form tissues.
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Proteoglycans01:05

Proteoglycans

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Glycans, a class of complex heterogeneous molecules, can be covalently attached to proteins to form glycosylated proteins that regulate various physiological and pathological processes. Glycosylated proteins or glycoproteins comprise N-linked and O-linked oligosaccharides. O-glycosylation is the most common type of protein glycosylation. Here, glycans attach to the oxygen atom of the hydroxyl groups of Serine or Threonine residues. O-linked glycosylation occurs later in protein processing,...
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Affinity and Avidity01:41

Affinity and Avidity

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Overview
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Immunoglobulin-like Cell Adhesion Molecules01:31

Immunoglobulin-like Cell Adhesion Molecules

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Immunoglobulin-like cell adhesion molecules or Ig-CAMs are a versatile group of cell surface glycoproteins belonging to the immunoglobulin protein superfamily. Ig-CAMs possess the characteristic immunoglobulin protein domains and other domains such as the fibronectin type III domain. The Ig domains are glycosylated to varying degrees in different Ig-CAMs.
Ig-CAMs exhibit either homophilic binding (to other Ig-CAMs) or heterophilic binding (to other ligands such as integrins). While most Ig-CAMs...
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Conjugated Proteins02:50

Conjugated Proteins

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Simple proteins and protein complexes contain only amino acids. In contrast, many other proteins, called conjugated proteins, covalently bond with non-protein moieties.
Nucleoproteins are protein complexes that contain nucleic acids, categorized as deoxyribonucleoproteins (DNPs) or ribonucleoproteins (RNPs) respectively. The nucleosome is a typical example of a DNP where nuclear DNA is associated with histone proteins. The major antigen for the Covid-19 virus SARS-CoV is an RNP that is critical...
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Protein Glycosylation01:25

Protein Glycosylation

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Glycosylation, the most common post-translational modification for proteins, serves diverse functions. Adding sugars to proteins makes the proteins more resistant to proteolytic digestion. Glycosylated proteins can act as markers and receptors to promote cell-cell adhesion. Additionally, they have many essential quality control functions in the cell, such as correct protein folding and facilitating transport of misfolded proteins to the cytosol, which can be degraded.
Glycosylation occurs in...
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相关实验视频

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Expression of Exogenous Antigens in the Mycobacterium bovis BCG Vaccine via Non-genetic Surface Decoration with the Avidin-biotin System
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(Strept) 亚维丁结合物 结合物 糖结合物

Nadezhda V Shilova1,2, Svetlana M Polyakova3, Alexey Yu Nokel3,2

  • 1Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia. pumatnv@gmail.com.

Biochemistry. Biokhimiia
|December 8, 2024
PubMed
概括

(Strept) 常用于测试中的avidins,也与血型抗原等甘氨酸结合. 这种不特定的甘氨酸结合,需要多价性,可能会导致碳水化合物分析中的错误,并需要仔细控制 (链状) 丁度.

关键词:
艾维迪尼 艾维迪尼葡萄糖糖是什么 葡萄糖是什么葡萄糖合物 葡萄糖合物微阵列是微阵列中的一个.印刷的糖甘阵列列.在Streptavidin中使用.

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科学领域:

  • 生物化学 生化学
  • 免疫学 免疫学 免疫学
  • 葡萄糖生物学 葡萄糖生物学

背景情况:

  • (Strept) 丁是广泛用于免疫学试验和组织化学检测生物化分子的试剂.
  • 它们的主要功能依赖于高亲和度与生物素的结合.

研究的目的:

  • 为了研究超越生物素 (strept) avidins的潜在的非特异性结合相互作用.
  • 确定这些相互作用的性质及其在生物样本分析中的影响.

主要方法:

  • 使用光标记和合 (链状) 胺.
  • 测试了对各种甘氨酸的结合,包括哺乳动物甘氨酸蛋白和甘油脂片段 (例如,ABO血型抗原,橄酸胺,6-O-硫酸寡糖).
  • 使用不同度的单体和聚合物甘氨酸合物,评估了甘氨酸结合的抑制.

主要成果:

  • 已经证明,除了生物素外, (链条) 亚维丁还与特定的甘氨酸结合,包括 ABO 血型抗原和硫化寡糖,以及生物素.
  • 这种糖甘结合活性是多价值依赖的,被聚合物糖甘结合物抑制,但在微分子度下不是单质的.
  • 这些发现突出了涉及复杂碳水化合物结构的分析中文物的潜在来源.

结论:

  • (链条) 亚维丁与细胞表面甘氨酸的非特异性结合可以干扰免疫学测试和组织化学.
  • 研究人员应该注意这种相互作用,特别是在富含糖蛋白和糖脂质的样本中.
  • 在分析系统中最大限度地减少 (条形蛋白) 过量对于在分析含碳水化合物样本时防止错误结果至关重要.