维门和德斯中间丝维护了线粒体膜的潜力
Alexander A Dayal1, Olga I Parfenteva1, Wang Huiying1
1Institute of Protein Research, Russian Academy of Sciences, Moscow, 119334, Russia.
Biochemistry. Biokhimiia
|December 8, 2024
概括
这项研究表明,维门和德斯中间纤维 (IF) 对于维持细胞中的线粒体膜潜力至关重要. 破坏两者,但不是单个蛋白质,显著降低了这种潜力.
科学领域:
- 细胞生物学 细胞生物学
- 生物化学 生化学
- 细胞骨动力学 细胞骨动力学
背景情况:
- 介质纤维 (IFs) 是细胞骨的关键组成部分,提供机械支持和器官的定位.
- 德斯敏和维丁是分别在肌肉和介质细胞中发现的IF蛋白,可以形成混合网络.
- 虽然可以单独调节线粒体活动,但desmin和vimentin在线粒体功能中的联合作用尚不清楚.
研究的目的:
- 调查共表达的维门和德斯介质纤维在维护线粒体膜潜力的作用.
- 确定维门和德斯在线粒体功能中是否起着不同的或重叠的作用.
主要方法:
- 利用BHK21细胞进行工程,以表达维门和德斯中间体纤维.
- 采用基因淘汰和RNA干扰技术来选择性地抑制维门和/或desmin的表达.
- 测量了不同水平的维门和德斯表达的细胞中的线粒体膜潜力.
主要成果:
- 破坏单独维门或德斯的表达并没有显著改变线粒体膜潜力.
- 同时抑制维门和德斯导致线粒体膜潜能显著减少20%.
- 这些发现表明,这两种蛋白质在维护线粒体完整性方面具有协同作用或同等重要的作用.
结论:
- 维门和德斯中间体纤维对维持正常的线粒体膜潜能至关重要.
- 维门和德斯的联合存在和功能对于细胞线粒体健康至关重要.
- 这项研究强调了混合中间丝网在调节线粒体功能的重要性.
相关概念视频
The Inner Mitochondrial Membrane
3.3K
The inner mitochondrial membrane is the primary site of ATP synthesis. The inner membrane domain that forms a smooth layer adjacent to the outer membrane is called the inner boundary membrane. This domain contains membrane transporters that drive metabolites in and out of the mitochondria. In contrast, the inner membrane network that invaginates into the matrix space is called the cristae membrane. This domain accounts for principle mitochondrial function as it accommodates the protein...
3.3K
Disassembly of Intermediate Filaments
2.0K
Intermediate filaments (IFs) do not undergo spontaneous disassembly. Enzymes, kinases, and phosphatases add and remove phosphates from specific sites to regulate their disassembly. The IF concentration in the cytoplasm also regulates the disassembly. If the concentration crosses a threshold, it activates the protein kinases in the vicinity, allowing the phosphorylation of IFs.
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
Keratin proteins, found at the cell periphery near cell junctions, undergo a cycle of assembly and disassembly. In Type...
2.0K
Mitochondrial Membranes
8.6K
A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
8.6K
Types of Intermediate Filaments
3.6K
The intermediate filaments are an essential component of the cytoskeleton. Presently six types of intermediate filament have been identified. Type I and II are acidic and basic keratin proteins. Type III is of mesodermal origin and comprises four proteins: vimentin, desmin, glial fibrillary acidic protein (GFAP), and peripherin. Vimentin is commonly found in mesenchymal cells, desmin in muscle cells, GFAP in astrocytes, while peripherin is found in peripheral nervous system neurons (PNS). Type...
3.6K
The Structure of Intermediate Filaments
3.9K
The intermediate filaments are one of three widely studied cytoskeletal filaments. They are so named as their diameter (10 nm) is in between that of microfilaments (7 nm) and the microtubules (25 nm). These filaments are highly stable and can remain intact when exposed to high salt concentrations and detergents. These filaments are responsible for providing stability and mechanical support to the cells. They also help in cell adhesion and maintaining tissue integrity.
Intermediate...
Intermediate...
3.9K
Structure of Porins
2.9K
Mitochondria, chloroplasts, and gram-negative bacteria have transmembrane, beta-barrel proteins called porins to mediate the free diffusion of ions and metabolites across the membrane. Mitochondrial porin precursors contain conserved amino acid sequences called beta signals at their C-terminal. Beta signals have a motif of PoXGXXHyXHy (Po-Polar, X-Any amino acid, G-Glycine, Hy-LargeHydrophobic), which are crucial for precursor recognition to initiate precursor assembly. Beta-barrel...
2.9K


