可点击,热敏的水凝通过重组蜘蛛丝蛋白和间化学来实现持续的神经营养素的递送
Yue Li1, Chao Yang2, Shiyu Fang1
1Department of Chemical and Biological Engineering, The Hong Kong University of Science and Technology, Clear Water Bay, Kowloon, Hong Kong SAR, China.
Advanced materials (Deerfield Beach, Fla.)
|December 9, 2024
概括
一种由蜘蛛丝蛋白制成的新型可注射水凝,可持续提供神经再生的治疗蛋白质. 这种生物材料在视神经受伤后促进了轴突的再生和神经元的生存.
科学领域:
- 生物材料科学 生物材料科学
- 再生医学是一种再生医学.
- 神经科学是一个神经科学.
背景情况:
- 提供慢性疾病的蛋白质疗法需要最少的侵入性,持续的方法,避免病毒载体.
- 中枢神经系统的再生,特别是受伤后的轴突再生,仍然是一个重大的临床挑战.
研究的目的:
- 开发一种可注射,热敏的水凝系统,使用复合蜘蛛丝蛋白 (spidroin) 进行持续的蛋白质治疗.
- 用生物活性基因功能化水凝,以增强神经保护和再生.
主要方法:
- 一种重组的spidroin溶液被设计为通过超声波在37°C进行sol-gel过渡,从而实现可注射性.
- 使用SpyTag/SpyCatcher化学物质将神经变因子 (如状神经变因子) 和细胞结合联体结合到蜘蛛水凝中.
- 在临床前的模型中,在视神经受伤后,用 CNTF 的功能化水凝进行了静脉内注射.
主要成果:
- 蜘蛛系统展示了热触发的凝,提供了可注射和稳定的平台.
- 通过SpyTag/SpyCatcher化学与CNTF的功能化成功创建了一个神经支持生物材料.
- 玻璃内注射导致持续的JAK/STAT3信号传递,改善神经元的存活率,以及视神经损伤后显著的轴突再生.
结论:
- 一种多功能,可注射的基于蜘蛛丝的水凝系统已成功开发,用于持续的蛋白质输送.
- 这种生物材料平台显示出促进神经保护和再生的巨大潜力,为病毒传递系统提供了一个有希望的替代方案.
- 该系统的通用性表明在治疗需要蛋白质治疗的慢性伤害和疾病方面具有广泛的应用.
相关概念视频
01:35Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre- and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
01:25Aquaporins
Aquaporins or AQPs are a family of integral membrane proteins whose primary function is to transport water, while some called aquaglyceroporins also transport glycerol. In addition, aquaporins have also been suspected to be involved in transporting volatile substances, such as carbon dioxide and ammonia, across membranes. Such AQPs that act as gas channels are often highly expressed in cells involved in the gaseous exchange, such as red blood cells, epithelial cells, and pulmonary capillaries.
01:28Electrical Synapses
Electrical synapses found in all nervous systems play important and unique roles. In these synapses, the presynaptic and postsynaptic membranes are very close together (3.5 nm) and are actually physically connected by channel proteins forming gap junctions.
Gap junctions allow the current to pass directly from one cell to the next. In contrast, in the chemical synapse, the neurotransmitters carry the information through the synaptic cleft from one neuron to the next. They consist of two...
Gap junctions allow the current to pass directly from one cell to the next. In contrast, in the chemical synapse, the neurotransmitters carry the information through the synaptic cleft from one neuron to the next. They consist of two...
01:25Long-term Potentiation
Long-term potentiation, or LTP, is one of the ways by which synaptic plasticity—changes in the strength of chemical synapses—can occur in the brain. LTP is the process of synaptic strengthening that occurs over time between pre and postsynaptic neuronal connections. The synaptic strengthening of LTP works in opposition to the synaptic weakening of long-term depression (LTD) and together are the main mechanisms that underlie learning and memory.
Hebbian LTP
LTP can occur when presynaptic neurons...
Hebbian LTP
LTP can occur when presynaptic neurons...