Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Antimicrobial Mechanism of Temporin L Peptide Against Bacillus cereus from Fermented Sausage.

Probiotics and antimicrobial proteins·2026
Same author

Frequent exposure to biologics is associated with small intestinal bacterial overgrowth in patients with Crohn's disease: a retrospective case-control study.

PeerJ·2026
Same author

Clinical value of repeat kidney biopsy in pediatric IgA vasculitis nephritis.

Pediatric nephrology (Berlin, Germany)·2026
Same author

Processing-dependent hepatic risk and benefit of Polygonum Multiflorum Radix: Integrated chemical profiling, in vivo validation and candidate quality control markers.

Journal of pharmaceutical and biomedical analysis·2026
Same author

Intratumor <i>Lactobacillus</i> drives ferroptosis resistance via D-lactate-STAT3 K631 lactylation in esophageal squamous cell carcinoma.

Gut microbes·2026
Same author

From Molecular Recognition to On-Site Detection: A Highly Selective Strategy for Monitoring Gizzerosine in Feed by Overcoming Histamine Interference.

Journal of agricultural and food chemistry·2026
Same journal

Impact of an Artificial Albumin Corona on Surface Charge-Driven Nano-Bio Interactions and Cytotoxicity of Silver Nanoparticles.

ACS omega·2026
Same journal

Structural and Functional Disruption of Thiopurine S‑Methyltransferase by the A80P Variant: A Simulation and Genotyping Study.

ACS omega·2026
Same journal

CRISPR/Cas12a2-Mediated Ultrasensitive Assay for Rapid Detection of H1N1 Influenza Virus RNA.

ACS omega·2026
Same journal

Photocatalytic Treatment of Real Sugar Industry Wastewater Using Lignocellulosic Biomass-Derived Hydrochar/g-CN.

ACS omega·2026
Same journal

Electrochemical Dopamine Biosensor Based on Plant-Derived Peroxidase Immobilized on Titanate Nanowires.

ACS omega·2026
Same journal

Revealing the Effects of Process Parameters on Structural, Thermal, Mechanical, Biodegradation, and Biocompatibility Properties on the Electrospinning of Poly(vinyl alcohol)/Microbial Inulin Nanofibers.

ACS omega·2026
查看所有相关文章

相关实验视频

Updated: Jun 5, 2025

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
09:12

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology

Published on: May 3, 2021

2.5K

机器学习驱动的方法用于纳米体亲和力预测.

Hua Feng1,2, Xuefeng Sun1, Ning Li3

  • 1Institute for Animal Health, Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, 116 Huayuan Road, Zhengzhou 450002, China.

ACS omega
|December 9, 2024
PubMed
概括
此摘要是机器生成的。

机器学习模型通过分析非共价相互作用来预测纳米体-连接物亲和力. 这种方法有助于开发纳米体 (Nbs) 用于研究和治疗应用.

更多相关视频

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
08:10

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

11.9K
Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.7K

相关实验视频

Last Updated: Jun 5, 2025

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology
09:12

Selection of Transporter-Targeted Inhibitory Nanobodies by Solid-Supported-Membrane SSM-Based Electrophysiology

Published on: May 3, 2021

2.5K
A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example
08:10

A Liquid Phase Affinity Capture Assay Using Magnetic Beads to Study Protein-Protein Interaction: The Poliovirus-Nanobody Example

Published on: May 29, 2012

11.9K
Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions
06:50

Author Spotlight: A Computational Approach to Decipher Amino Acid Preferences in Multispecific Protein-Protein Interactions

Published on: January 26, 2024

1.7K

科学领域:

  • 生物技术是生物技术.
  • 计算生物学 计算生物学
  • 免疫学 免疫学 免疫学

背景情况:

  • 纳米体 (Nbs) 具有很高的亲和力,特异性和稳定性,使它们在生物研究中具有价值.
  • 获得高亲和度Nbs的实验方法具有挑战性和耗时.
  • 通过计算预测Nb-联体相互作用可以简化Nb的开发.

研究的目的:

  • 为了比较机器学习算法来预测纳米体-连接体亲和力.
  • 为了确定影响Nb-配体结合的关键非共价相互作用.
  • 开发一个用于Nb查和设计的预测工具.

主要方法:

  • 评估了12个机器学习算法,以寻找Nb-ligand亲和力和八个非对应相互作用之间的模式.
  • 选择并优化了四个单独的模型 (SVMrB,RotFB,RFB,C50B) 和两个堆叠的模型 (StackKNN,StackRF).
  • 分析的特征的重要性,以确定关键的非共价相互作用.

主要成果:

  • 优化模型实现了大约0.70准确度和高特异性.
  • SVMrB,C50B和StackKNN有效地预测了高特异性 (>0.92) 的非亲属性Nbs.
  • 鉴定出结合和芳香相互作用是Nb-配体亲和力的关键决定因素.

结论:

  • 开发了一种新的计算工具,用于预测纳米体-连接物亲和力.
  • 该工具可以提高纳米体查和设计的效率.
  • 加快开发基于纳米体的药物和应用程序.