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Updated: Jun 5, 2025

Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems
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Rapid, Enzymatic Methods for Amplification of Minimal, Linear Templates for Protein Prototyping using Cell-Free Systems

Published on: June 14, 2021

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高度重复或低复杂度序列DNA的可靠放大,通过超酶介导的同热放大来实现

Jimin Kang, Fahad Rashid, Peter J Murray

    bioRxiv : the preprint server for biology
    |December 9, 2024
    PubMed
    概括

    这项研究介绍了SHARP (SSB-Helicase辅助快速PCR),一种使用超酶取代热循环的异热DNA放大方法. SHARP成功地放大了具有挑战性的DNA序列,包括重复性和高AT含量区域,用于克隆和基因编辑的应用.

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    Linear Amplification Mediated PCR – Localization of Genetic Elements and Characterization of Unknown Flanking DNA

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