相关实验视频

Updated: Jun 5, 2025

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase
07:27

Direct Restart of a Replication Fork Stalled by a Head-On RNA Polymerase

Published on: April 29, 2010

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TTF2促进了在线粒分裂中从停滞的分叉中进行复杂体驱逐

Geylani Can1,2,3, Maksym Shyian3, Archana Krishnamoorthy1,2

  • 1Department of Cell Biology, Blavatnik Institute, Harvard Medical School, Boston, MA 02115, USA.

bioRxiv : the preprint server for biology
|December 9, 2024
PubMed
概括

带有未复制DNA的甲基细胞面临着基因组的不稳定性. TRAIP由环素B-CDK1酸化,并由TTF2绑定,分解停滞的DNA复制体,确保染色体的稳定性.

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Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy12:04

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Published on: June 24, 2019

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Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System11:19

Inducing a Site Specific Replication Blockage in E. coli Using a Fluorescent Repressor Operator System

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Restarting Stalled Replication Forks02:37

Restarting Stalled Replication Forks

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Translesion (TLS) polymerases rescue stalled DNA polymerases at sites of damaged bases by replacing the replicative polymerase and installing a nucleotide across the damaged site. Doing so, TLS allows additional time for the cell to repair the damage before resuming regular DNA replication.
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In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...
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