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Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
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Nuclear reprogramming is the process of switching gene expression of one cell type to that of another cell type, usually from a differentiated cell state to an undifferentiated cell state. Differentiation occurs during processes such as development and morphogenesis, tissue regeneration, and malignancy. Cells can also be artificially induced to reprogram their gene expression by techniques such as nuclear transfer, induced pluripotency, and cell fusion. Such techniques have many applications in...
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使用CellREADR对RNA可编程细胞进行向和操纵.

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    此摘要是机器生成的。

    细胞READR是一种使用RNA传感来控制细胞功能的新技术. 这种可编程系统可以轻松监控和操纵动物细胞,为生物学和医学开辟了新的途径.

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    科学领域:

    • 分子生物学分子生物学
    • 生物技术是生物技术.
    • 遗传学 遗传学 是一个

    背景情况:

    • 开发精确且可扩展的方法来访问特定的动物细胞类型对于生物和医学研究至关重要.
    • 现有的技术往往缺乏广泛应用所需的多功能性和易于实施.

    研究的目的:

    • 介绍和详细介绍CellREADR (通过内源ADAR感应RNA传感进入细胞) 技术.
    • 为在细胞培养和动物模型中实施CellREADR提供一个全面的协议.
    • 展示基于RNA的系统在细胞监测和操纵方面的潜力.

    主要方法:

    • 设计和制造具有特定RNA结合区域和效应器有效载荷的CellREADRRNA传感器执行器设备.
    • 使用ADAR (作用于RNA的腺氨酸脱氨酶) 编辑机制,对效应蛋白进行条件翻译.
    • 在哺乳动物细胞培养和基于AAV的输送中验证实行协议,用于体内研究,特别是脑组织.

    主要成果:

    • 在2-3周内成功设计和验证了哺乳动物细胞培养中的CellREADR.
    • 在动物模型 (6-8周) 中证明AAV介导的体内输送和CellREADR的表征.
    • 已建立的最佳实践,实验控制和故障排除用于CellREADR实施.

    结论:

    • 细胞READR提供了一个多功能,简单和可通用的平台,用于基于RNA的动物细胞监测和操纵.
    • 该技术利用内生ADAR活动进行可编程控制,适用于各种组织和物种.
    • 该协议促进了CellREADR在基础研究和治疗开发中的广泛应用.