一个无标签的色度测量感应传感器,用于快速检测 leuco-malachite 绿色和 leuco-crystal 紫色的多重体
Runze Zhang1, Kexin Liu1, Fangran Jia1
1Department of Food and Biological Sciences, Agricultural College, Yanbian University, Yanji 133002, China. yjyang@ybu.edu.cn.
Analytical methods : advancing methods and applications
|December 9, 2024
概括
一种新的双特异性口味传感器可以快速,无标签地检测水产品中的共污染物白绿 (LMG) 和白晶紫 (LCV). 这一突破通过同时识别这些有害物质,提高了食品安全.
科学领域:
- 分析化学 分析化学
- 生物传感器是一种生物传感器.
- 食品安全 食品安全
背景情况:
- 藻绿 (LMG) 和藻水晶紫 (LCV) 是普遍存在的水生共污染物,对人类健康构成重大风险.
- 为了有效监测水产品,迫切需要快速和同时检测LMG和LCV的方法.
研究的目的:
- 开发一种能够识别LMG和LCV.的新型双特异性吸收体 (A5b).
- 为在水生样本中多重检测LMG和LCV而创建一个无标签的色度测量感应传感器.
主要方法:
- 一个双特异性阿巴体 (A5b) 的表征,其解离常数 (Kd) 为LMG的2.03 ± 0.13 μM,为LCV的0.97 ± 0.06 μM.
- 使用金纳米颗粒 (AuNPs) 和多二二甲基化物 (PDDA) 的色度感应传感器的开发.
- 胃感应器依赖于PDDA诱导的AuNPs聚合,这种聚合因存在与A5b结合的LMG或LCV而受到抑制.
主要成果:
- 胃感应器在增加LMG/LCV度时显示出可见的颜色变化,从红色到蓝色.
- 在1-6μM (LCV) 和1-8μM (LMG) 的线性范围内,达到77.9nM的LCV和123.1nM的LMG检测极限 (LOD).
- 成功验证了传感器在真实Crucian样本中的性能,表明其实际可用性.
结论:
- 这项研究报告了第一个用于同时检测LMG和LCV的双特异亲和探针.
- 一种灵敏,选择性和无标签的色度测量感应传感器已成功开发用于LMG和LCV的多重检测.
- 开发的吸食传感器对水产品中这些共同污染物的常规监测具有重大潜力.
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