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相关概念视频

Structure and Function of Platelets01:18

Structure and Function of Platelets

988
The cell fragments known as platelets are disc-shaped, with an average diameter of about 3 μm and a thickness of roughly 1 μm. They play a crucial role in the body's vascular clotting system, which also involves plasma proteins, blood cells, and blood vessel tissues.
Platelets are continually replenished, circulating in the bloodstream for 9-12 days before being removed by phagocytes, primarily in the spleen. A microliter of circulating blood contains between 150,000 and 450,000...
988

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流细胞计分析人类血小板组织因子表达的分析.

Marta Brambilla1, Alessia Becchetti1, Kevin Nallio1

  • 1Centro Cardiologico Monzino IRCCS.

Journal of visualized experiments : JoVE
|December 9, 2024
PubMed
概括

这项研究提出了一个标准化的流细胞测量协议,以准确测量组织因子 (TF) 阳性血小板,这对于在临床环境中评估血栓形成风险至关重要. 该方法量化了来自全血或血的静止和激活血小板的细胞内和表面TF.

科学领域:

  • 血液学 血液学 血液学
  • 免疫学 免疫学 免疫学
  • 生物技术是生物技术.

背景情况:

  • 组织因子 (TF) 是血液凝固和血栓形成的关键启动因子.
  • TF在人体血小板的一个子集中被细胞内存储,并在激活时暴露.
  • 增加的TF阳性血小板与前血栓状况相关,但它们的准确测量具有挑战性.

研究的目的:

  • 为量化TF阳性血小板建立一个标准化的流细胞测量协议.
  • 为了能够对各种血小板制剂中的细胞内和表面TF进行可靠的评估.
  • 为了解决TF阳性血小板评价过去的争议.

主要方法:

  • 关于全血,富血小板血 (PRP) 和洗净血小板中的TF阳性血小板的流动细胞计分析的详细协议.
  • 包括采样,制备,细胞刺激,标记,固定和透的程序.
  • 关于流细胞计设置的具体指南,用于准确识别血小板群体和TF事件分析.

主要成果:

  • 该协议允许在休息和激活条件下量化TF-阳性血小板.
  • 它区分了细胞内和表面的TF表达.
  • 该方法适用于全血和单独的血小板分量,并考虑PRP制备.

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结论:

  • 一个标准化,详细的协议测量TF-阳性血小板使用流细胞计已经开发.
  • 该方法为评估血小板相关的TF提供了可靠的工具,有助于评估血栓形成风险.
  • 该协议涉及预分析变量,并为准确量化提供了全面的步骤.