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含有托尔类受体激活剂和整体蛋白结合序列的脂的化

Valeria Castelletto¹, Lucas R de Mello¹, Jani Seitsonen²

¹School of Chemistry, Food Biosciences and Pharmacy, University of Reading, Whiteknights, Reading RG6 6AD, U.K.

ACS applied materials & interfaces

|December 9, 2024

概括

这项研究探讨了生物材料的脂自我组装. 不同的序对聚合有影响,对材料特性和细胞相容性有影响.

科学领域:

生物材料科学生物材料科学
超分子化学超分子化学
生物物理学的生物物理.

背景情况:

简短的生物活性对于开发先进生物材料至关重要.
结合序列和脂质链的脂具有独特的自我组装特性.
了解自我组装是控制生物材料功能的关键.

研究的目的:

研究含有特定生物活性序列的脂的自我组装行为 (CSK4收费类受体激动剂和RGDS整合素结合动机).
为了比较C16-CSK4RGDS的聚合特征与控制脂 (C16-CSK4GRDS) 具有编码序列.
批判性地评估不同的方法来确定关键菌度 (CMC),并了解差异.

主要方法:

微角X射线散射 (SAXS) 和冷传输电子显微镜 (cryo-TEM) 用于表征菌形成.
原子分子动力学 (MD) 模拟以建模自我组装.
光探针测定 (ANS,尼罗河红色),表面张力测量,电导率,UV/Vis光谱和圆形二元化 (CD) 探测聚合和分子构造.
使用纤维细胞体和肌细胞体进行细胞毒性测定.

主要成果:

这两种脂都能自组装成.
在不同的测量技术之间观察到CMC值的显著差异,特别是使用ANS光.
由于与阴离子残留结合的ANS光干扰被确定为异常结果的来源,CD光谱学证实了这一点.
混合序列脂 (C16-CSK4GRDS) 显示出更大的聚合倾向和协会数.
这两种脂在低度时显示出细胞相容性,但在高度时显示出细胞毒性.

结论:

证实了脂质的自我组装成小胞,但测量方法显著影响观察到的临界小胞度.
光探针与带电残留物的相互作用可能导致错误的CMC确定.
序列的微妙变化可以深刻地影响脂聚合行为和特性.
这些发现对于设计基于自组装脂的功能生物材料至关重要.

关键词:

聚合方式聚合方式聚合方式生物表面活性剂生物表面活性剂细胞相容性细胞相容性脂类是脂类中的一种.迈塞尔斯 (Micelles) 是一个非常有价值的人.分子动力学分子动力学

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