在喀麦隆的Candida krusei (Pichia kudriavzevii) 多部位序列类型和抗真菌敏感性概况
Cyrille Levis Kountchou1,2, Jihane Kabtani3, Aude Ngueguim Dougue4
1Center for Medical Research in Health and Priority Pathologies, Institute of Medical Research and Medicinal Plants Studies, Yaoundé, 13033, Cameroon.
概括
导致感染的真菌Candida krusei在喀麦隆的艾滋病毒患者中显示出有关抗真菌的耐药性. 大多数隔离物表现出对多个抗真菌类的耐药性,突出显示了监测的必要性.
科学领域:
- 医学真菌学 医学真菌学
- 传染性疾病 传染性疾病
- 分子流行病学分子流行病学
背景情况:
- 菌 (Candida krusei) 是一种重要的真菌病原体,以其对可纳的内在耐药性而闻名.
- 携带人类免疫缺陷病毒 (HIV) 的患者特别容易感染侵入性真菌感染.
研究的目的:
- 通过使用多位序列类型 (MLST) 来分析喀麦隆艾滋病毒阳性患者的Candida krusei分离物种群体结构.
- 评估这些C. krusei分离物体的体外抗真菌敏感性概况.
主要方法:
- 使用标准实验室方法和MALDI-TOF MS.识别C. krusei.
- 通过MLST进行种群结构分析,识别二倍体序列类型 (DST).
- 使用Sensititre Yeast OneTM微稀释方法进行抗真菌敏感性测试.
主要成果:
- 在43个C. krusei分离物中发现了32种不同的DST,其中31种是新型.
- 很大一部分 (32.6%) 呈现出对至少三个抗真菌类别的耐药性或非野生类型的表型.
- 在抗真菌最小抑制度 (MICs) 中观察到内隔离和地理异质性.
结论:
- 这项研究揭示了喀麦隆艾滋病毒阳性个体中C. krusei的多样化人口结构.
- 广泛的抗真菌耐药性需要强大的分子流行病学和监测策略对C. krusei.
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