玉米丝提取物的质量控制使用红外光谱以及统计方法
Aiko Sano1, Yutaka Inoue1, Yuji Higuchi2
1Faculty of Pharmacy and Pharmaceutical Sciences, Josai University, 1-1 Keyakidai, Sakado, Saitama, 3500295, Japan.
概括
玉米丝水性提取物具有抗糖化特性,活性成分是素. 减弱总反射率-里埃变换红外光谱学与多变量分析相结合,提供了一种可靠的方法来控制这些提取物的质量.
科学领域:
- 分析化学 分析化学
- 自然产品化学 自然产品化学
- 频谱学是一种光谱学.
背景情况:
- 玉米丝水性提取物具有糖化抑制活性,归因于素.
- 宁作为一种潜在的标记化合物,用于对玉米丝提取物的质量控制.
- 像HPLC这样的传统方法不适合分析这些提取物中的素,因为它具有宏分子性质.
研究的目的:
- 开发和验证一种用于评估玉米丝水性提取物的抗糖化活性的新方法.
- 建立玉米丝提取物使用素作为标记物的质量控制措施.
- 为了探索减弱总反射率 - 里埃变换红外光谱 (ATR-FTIR) 与多变量分析相结合的实用性.
主要方法:
- 使用ATR-FTIR光谱分析玉米丝水性提取物.
- 应用多变量统计分析,特别是主要成分回归 (PCR) 和部分最小平方回归 (PLSR),用于光谱数据分析.
- 与糖化抑制活性相关的光谱数据,以构建预测模型.
主要成果:
- 该PCR模型显示了高可预测性,R2 = 0.981和RMSECV = 2.356.
- 使用特定光谱区域的PLSR模型,实现了优异的预测准确度,R2 = 0.994和RMSECV = 1.325%.
- 与多变量校准相结合的ATR-FTIR光谱学在评估玉米丝水性提取物的抗糖化活性方面被证明是有效的.
结论:
- 具有多变量校准的ATR-FTIR光谱法是一种可靠和准确的方法,用于对玉米丝水性提取物进行质量控制.
- 这种技术可以对抗糖化活性进行可靠的评估,促进玉米丝作为功能性食品和化品成分的使用.
- 开发的方法克服了传统分析技术的局限性,用于分析像素这样的宏分子成分.
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